Abstract
The major cause of cancer-related deaths in patients with lung adenocarcinoma (LAD) is due to distant metastasis. Many reports have indicated that miRNA plays a key role in tumour metastasis. The expression of miR-197 is correlated with LAD progression, however, the mechanism of miR-197 is still unknown in the processing of LAD. A Boyden chamber migration/invasion assay was used for the metastatic function study in vitro. Real-time PCR and Western blot assays were employed to analyse the EMT hallmark changes in both the mRNA and protein levels. \(3^{\prime }\)-UTR reporter luciferase assay was used to show that HIPK2 is a direct target of miR-197. miR-197 enhances LAD cell migration and invasion miR-197. The downregulation of miR-197 suppresses the EMT and migration ability. HIPK2 is a direct functional target of miR-197 in LAD metastasis. In summary, miR-197 controls EMT and metastasis by directly silencing HIPK2. The findings suggest that interfering with the miR-197-dependent regulation of HIPK2 could be a useful approach for the treatment of patients with late stage metastatic LAD.
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Corresponding editor: Dhavendra Kumar
NZ carried out most of the experiments and drafted the manuscript. LT carried out migration and invasion assays. ZM designed the study and performed the statistical analysis. NG conceived the study, and designed and coordinated, and helped to draft the manuscript. All authors read and approved the final manuscript.
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Zhang, N., Tian, L., Miao, Z. et al. MicroRNA-197 induces epithelial–mesenchymal transition and invasion through the downregulation of HIPK2 in lung adenocarcinoma. J Genet 97, 47–53 (2018). https://doi.org/10.1007/s12041-018-0881-4
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DOI: https://doi.org/10.1007/s12041-018-0881-4