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Mitochondrial DNA sequence variation in Drosophilid species (Diptera: Drosophilidae) along altitudinal gradient from Central Himalayan region of India

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Abstract

Central Himalayan region of India encompasses varied ecological habitats ranging from near tropics to the mid-elevation forests dominated by cool-temperate taxa. In past, we have reported several new records and novel species from Uttarakhand state of India. Here, we assessed genetic variations in three mitochondrial genes, namely, 16S rRNA, cytochrome c oxidase subunit I and cytochrome c oxidase subunit II (COI and COII) in 26 drosophilid species collected along altitudinal transect from 550 to 2700 m above mean sea level. In the present study, overall 543 sequences were generated, 82 for 16S rRNA, 238 for COI, 223 for COII with 21, 47 and 45 mitochondrial haplotypes for 16S rRNA, COI and COII genes, respectively. Almost all species were represented by 2–3 unique mitochondrial haplotypes, depicting a significant impact of environmental heterogeneity along altitudinal gradient on genetic diversity. Also for the first time, molecular data of some rare species like Drosophila mukteshwarensis, Liodrosophila nitida, Lordiphosa parantillaria, Lordiphosa ayarpathaensis, Scaptomyza himalayana, Scaptomyza tistai, Zaprionus grandis and Stegana minuta are provided to public domains through this study.

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Acknowledgements

The authors greatly acknowledge University Grants Commission, New Delhi, for granting BSR fellowship to Manisha Sarswat and major research project to RSF (F. no. 37-198/2009 {SR}) and Department of Science and Technology, New Delhi, for granting INSPIRE fellowship to Saurabh Dewan. We also acknowledge Dr Mukul Kandpal for providing some samples and National Biodiversity Authority, Chennai, India.

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  1. Cytogenetics and Molecular Systematics Laboratory, UGC-SAP Department of Zoology and Biotechnology, HNB Garhwal University, Garhwal, 246 174, India

    MANISHA SARSWAT, SAURABH DEWAN & RAJENDRA SINGH FARTYAL

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  1. MANISHA SARSWAT
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Correspondence to RAJENDRA SINGH FARTYAL.

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[Sarswat M., Dewan S. and Fartyal R. S. 2016 Mitochondrial DNA sequence variation in Drosophilid species (Diptera: Drosophilidae) along altitudinal gradient from central Himalayan region of India. J. Genet. 95, xx–xx]

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SARSWAT, M., DEWAN, S. & FARTYAL, R.S. Mitochondrial DNA sequence variation in Drosophilid species (Diptera: Drosophilidae) along altitudinal gradient from Central Himalayan region of India. J Genet 95, 357–367 (2016). https://doi.org/10.1007/s12041-016-0646-x

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