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Electrochemical and spectroscopic studies of the interaction of (+)-epicatechin with bovine serum albumin

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Abstract

The mechanism of the electrochemical oxidation of (+)-epicatechin (epiCAT) in a hydro-alcoholic medium, at a glassy carbon electrode, was studied using cyclic and square wave voltammetric techniques and at different pHs. It proceeds in a cascade mechanism related to the two resorcinol hydroxyl groups and the other three hydroxyl groups in epiCAT which present electroactivity and is pH-dependent. The oxidation of the 3′,4′-dihydroxyl moiety, occurs first at a very low positive potential, and it is a two-electron/two proton reversible reaction. The product obtained from the oxidation of the 3′,4′-dihydroxyl moiety then undergoes a chemical reaction followed by an electrochemical process to give an electroinactive product. The proposed mechanism is an ECE type mechanism. After the addition of bovine serum albumin (BSA) to epiCAT solution, the oxidation peak currents decreased with no peak potential shift and no new peaks appeared. The diffusion coefficients of both free and bound epiCAT were estimated from the cyclic voltammetry data (Df = 2.37 × 10−10 cm2s−1 and Db = 6.28 × 10−11 cm2s−1). An epiCAT-BSA complex is formed with binding constant Kapp = 1.8 × 104, calculated from UV-vis spectroscopy data.

Graphical abstract

The oxidation process of (+)-epicatechin (I to I’ and II) in a hydro-alcoholic medium at a glassy carbon electrode follows an ECE type mechanism.

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Abbreviations

BSA:

Bovine serum albumin

epiCAT:

(+)-Epicatechin

SCE:

Saturated calomel electrode

CV:

Cyclic voltammetry

SWV:

Square wave voltammetry

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Acknowledgements

The authors thank Professor Pierre Mkounga (Laboratory of Structural and Analytical Chemistry, Faculty of Science, University of Yaoundé 1, Cameroon) for providing epicatechin used in this study. The authors also thank Dr Xavier Siwe Noundou for patiently proofreading the draft manuscript.

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Authors and Affiliations

  1. Laboratoire de Chimie Minérale, Faculté des Sciences, Université de Douala, B.P. 24157, Douala, Cameroun

    Jules-Blaise Mabou Leuna & Achille Nassi

  2. Laboratoire de Chimie Analytique, Faculté des Sciences, Université de Yaoundé 1, B.P. 812, Yaoundé, Cameroun

    Jules-Blaise Mabou Leuna, Martin Pengou, Sergeot Delor Kungo Sop, Charles Péguy Nanseu-Njiki & Emmanuel Ngameni

  3. Département de Chimie, Faculté des Sciences, Université de Maroua, B.P. 814, Maroua, Cameroun

    Martin Pengou & Francis Merlin Melataguia Tchieno

  4. Département de Chimie, Faculté des Sciences, Université de Dschang, B.P. 67, Dschang, Cameroun

    Francis Merlin Melataguia Tchieno

  5. Département de Chimie, Faculté des Sciences, Université de Sherbrooke, 2500 Boulevard de l’Université, Sherbrooke, J1K 2R1, Canada

    Achille Nassi

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  1. Jules-Blaise Mabou Leuna
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Correspondence to Jules-Blaise Mabou Leuna.

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Leuna, JB.M., Pengou, M., Tchieno, F.M.M. et al. Electrochemical and spectroscopic studies of the interaction of (+)-epicatechin with bovine serum albumin. J Chem Sci 133, 44 (2021). https://doi.org/10.1007/s12039-021-01894-6

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  • DOI: https://doi.org/10.1007/s12039-021-01894-6

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