Abstract
In this paper, a novel disposable immunosensor for the detection of α-fetoprotein (AFP) based on the Indium tin oxide (ITO) modified by the sequential electro-deposition of the nanogold particles (nano-Au) and prussian blue (PB) is described. The ITO is employed to reduce the cost, instead of expensive gold electrode, glassy carbon electrode or platinum electrode. The layer-by-layer (LBL) electro-deposition of the nano-Au, PB is used for blocking the possible leakage from the substrate electrode surface and to prevent shedding of composite membrane. Under optimal conditions, the proposed immunosensor displays a broad linear response to AFP, the working range being 0·25 to 300·0 ng mL−1 with a detection limit of 0·04 ng mL−1. The studied immunosensor exhibits high sensitivity, fast analytical time and good stability. The proposed methodology is potentially attractive for clinical immunoassays.
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Li, Y., Liang, WB., Fang, LC. et al. Disposable amperometric immunosensor based on layer-by-layer electro-depositing of the nanogold particles, prussian blue-modified indium tin oxide for determination of α-fetoprotein. J Chem Sci 121, 1069–1076 (2009). https://doi.org/10.1007/s12039-009-0121-9
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DOI: https://doi.org/10.1007/s12039-009-0121-9