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LncRNA-SNHG16 promotes proliferation and migration of acute myeloid leukemia cells via PTEN/PI3K/AKT axis through suppressing CELF2 protein

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Abstract

The silence of lncRNA small nucleolar RNA host gene 16 (SNHG16) suppressed acute lymphoblastic leukemia (ALL) cell proliferation and migration, whereas its role in acute myeloid leukemia (AML) still lacks clarity. This study showed that SNHG16 was upregulated in AML patients and cells. And SNHG16 overexpression remarkably enhanced the proliferation and migration capacities of HL60 and AML-193 cells, while SNHG16 knockdown acted the opposite way. Subsequently, we revealed that SNHG16 directly bound to CELF2 (CUGBP Elav-like family member 2) protein, and caused CELF2 mRNA unstably and proteins reducing. CELF2 was decreased both in AML patients and cells. CELF2 overexpression or interference weakened the effect of overexpressing or silencing SNHG16 on proliferation and migration. Moreover, the transfection of pcDNA-CELF2 elevated PTEN (phosphatase and tensin homolog) activity and hindered the phosphoinositide 3-kinase (PI3K)/AKT signaling. And SNHG16 reduced PTEN activity and promoted the PI3K/AKT pathway activation by restraining CELF2. Furthermore, GDC-0941 (a specific inhibitor of the PI3K/AKT pathway) impeded the effect of SNHG16 increase, and bpV(pic) (a specific PTEN inhibitor) declined the effect of SNHG16 decrease on cell proliferation and migration. Taken together, the present study indicated that SNHG16 promoted proliferation and migration of AML cells via PTEN/PI3K/AKT axis through suppressing CELF2 protein.

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Funding

This work was supported by the Shaanxi Key R & D Program Project (No. 2018SF-205).

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Authors and Affiliations

  1. School of Medicine, Xi’an Peihua University, 888 Changning Street, Xi’an, 710125, China

    Min Shi, Qi Wei & Lei Chen

  2. Clinical Laboratory, Xi’an Chang’an Hospital, 17 Wenjing Road, Xi’an, 710016, China

    Ruili Yang

  3. Department of Hematology, Xi’an Central Hospital, 161 Xiwu Road, Xi’an, 710003, China

    Jing Lin & Xiaobo Guo

  4. Department of Blood Transfusion, Xi’an Central Hospital, 161 Xiwu Road, Xi’an, 710003, China

    Weifeng Gong

  5. Clinical Laboratory, Xi’an Central Hospital, 161 Xiwu Road, Xi’an, 710003, China

    Yang Li

Authors
  1. Min Shi
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  2. Ruili Yang
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  3. Jing Lin
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  4. Qi Wei
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  5. Lei Chen
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  6. Weifeng Gong
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  7. Yang Li
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  8. Xiaobo Guo
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Corresponding author

Correspondence to Xiaobo Guo.

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Statement of Ethics

All patients and healthy volunteers gave their informed consent to the study and signed the informed consent form. This research was approved by the Ethics Committee of the Xi’an Central Hospital and adhered to the ethical guidelines of the Declaration of Helsinki.

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Corresponding editor: Sorab Dalal

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Shi, M., Yang, R., Lin, J. et al. LncRNA-SNHG16 promotes proliferation and migration of acute myeloid leukemia cells via PTEN/PI3K/AKT axis through suppressing CELF2 protein. J Biosci 46, 4 (2021). https://doi.org/10.1007/s12038-020-00127-1

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