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α2,6 Sialylation associated with increased β1,6-branched N-oligosaccharides influences cellular adhesion and invasion

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Abstract

Expression of β1,6-branched N-linked oligosaccharides have a definite association with invasion and metastasis of cancer cells. However, the mechanism by which these oligosaccharides regulate these processes is not well understood. Invasive variants of B16 murine melanoma, B16F10 (parent) and B16BL6 (highly invasive variant) cell lines have been used for these studies. We demonstrate that substitution of α2,6-linked sialic acids on multiantennary structures formed as a result of β1,6-branching modulate cellular adhesion on both extracellular matrix (ECM) and basement membrane (BM) components. Removal of α2,6 sialic acids either by enzymatic desialylation or by stably down-regulating the ST6Gal-I (enzyme that catalyses the addition of α2,6-linked sialic acids on N-linked oligosaccharides) by lentiviral driven shRNA decreased the adhesion on both ECM and BM components and invasion through reconstituted BM matrigel.

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Acknowledgements

We acknowledge Prof IJ Fidler for kindly providing the murine B16 melanoma cell lines and the support received from Mr Sanjay Bane for his help in some of the key experiments. We thank Ms Hemal Somaiya for cloning ST6Gal-I shRNA in pTRIPz vector and Mrs Rekha Gaur and Ms Shamal Vetale for acquisition of Flow cytometric data, and Mr Chavan and Mr Pawar for their technical support. We thank Council of Scientific and Industrial Research (CSIR), India, for their financial support towards the project and Department of Biotechnology (DBT), India, for the Junior and Senior Research Fellowships to AR.

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  1. Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Sector 22, Kharghar, Navi Mumbai, 410 210, India

    Amit Ranjan & Rajiv D Kalraiya

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  1. Amit Ranjan
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  2. Rajiv D Kalraiya
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Correspondence to Rajiv D Kalraiya.

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Corresponding editor: SUDHA BHATTACHARYA

Ranjan A and Kalraiya RD 2013 α2,6 Sialylation associated with increased β1,6-branched N-oligosaccharides influences cellular adhesion and invasion. J. Biosci. 38 1–10] DOI 10.1007/s12038-013-9382-z

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Ranjan, A., Kalraiya, R.D. α2,6 Sialylation associated with increased β1,6-branched N-oligosaccharides influences cellular adhesion and invasion. J Biosci 38, 867–876 (2013). https://doi.org/10.1007/s12038-013-9382-z

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  • DOI: https://doi.org/10.1007/s12038-013-9382-z

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