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Metabolic response to exogenous ethanol in yeast: An in vivo statistical total correlation NMR spectroscopy approach

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Abstract

In vivo NMR spectroscopy, together with selectively 13C-labelled substrates and ‘statistical total correlation spectroscopy’ analysis (STOCSY), are valuable tools to collect and interpret the metabolic responses of a living organism to external stimuli. In this study, we applied this approach to evaluate the effects of increasing concentration of exogenous ethanol on the Saccharomyces cerevisiae fermentative metabolism. We show that the STOCSY analysis correctly identifies the different types of correlations among the enriched metabolites involved in the fermentation, and that these correlations are quite stable even in presence of a stressing factor such as the exogenous ethanol.

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Authors and Affiliations

  1. Department of Pharmaceutical and Applied Chemistry, University of Siena, Siena, Italy

    Maso Ricci, Marianna Aggravi, Claudia Bonechi, Silvia Martini, Anna Maria Aloisi & Claudio Rossi

  2. Center for Colaff1loid and Surface Science (CSGI), Via della Lastruccia 3, Sesto Fiorentino, FI, Italy

    Maso Ricci, Marianna Aggravi, Claudia Bonechi, Silvia Martini, Anna Maria Aloisi & Claudio Rossi

  3. San Carlo Clinic, Via dell’Ospedale 21, 20037, Paderno Dugnano, MI, Italy

    Maso Ricci

  4. Pain and Stress Neurophysiology Lab, Department of Physiology, University of Siena, Siena, Italy

    Anna Maria Aloisi

Authors
  1. Maso Ricci
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  2. Marianna Aggravi
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  3. Claudia Bonechi
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  4. Silvia Martini
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  5. Anna Maria Aloisi
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  6. Claudio Rossi
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Corresponding author

Correspondence to Maso Ricci.

Additional information

Corresponding editor: Anant Bahadur Patel

MS received 10 January 2012; accepted 07 Jun /2012

Corresponding editor: ANANT BAHADUR PATEL

[Ricci M, Aggravi M, Bonechi C, Martini S, Aloisi AM and Rossi C 2012 Metabolic response to exogenous ethanol in yeast: An in vivo statistical total correlation NMR spectroscopy approach. J. Biosci. 37 1–7] DOI 10.1007/s12038-012-9237-z

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Ricci, M., Aggravi, M., Bonechi, C. et al. Metabolic response to exogenous ethanol in yeast: An in vivo statistical total correlation NMR spectroscopy approach. J Biosci 37, 749–755 (2012). https://doi.org/10.1007/s12038-012-9237-z

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  • DOI: https://doi.org/10.1007/s12038-012-9237-z

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