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Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue

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Abstract

The aim of this study was to propose the use of red light-emitting diode (LED) as an alternative light source for methylene blue (MB) photosensitizing effect in photodynamic therapy (PDT). Its effectiveness was tested against Staphylococcus aureus (ATCC 26923), Escherichia coli (ATCC 26922), Candida albicans (ATCC 90028) and Artemia salina. The maximum absorption of the LED lamps was at a wavelength of 663 nm, at intensities of 2, 4, 6 and 12 J.cm−2 for 10, 20, 30 and 60 min of exposure, respectively. Assays with and without LED exposure were carried out in plates containing MB at concentrations of 7 to 140.8 μM for microorganisms and 13.35 to 668.5 μM for microorganisms or microcrustaceans. The LED exposure induced more than 93.05%, 93.7% and 93.33% of growth inhibition for concentrations of 42.2 μM for S. aureus (D-value=12.05 min) and 35.2 μM for E. coli (D-value=11.51 min) and C. albicans (D-value=12.18 min), respectively after 20 min of exposure. LED exposure for 1 h increased the cytotoxic effect of MB against A. salina from 27% to 75%. Red LED is a promising light device for PDT that can effectively inhibit bacteria, yeast and microcrustacean growth.

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Abbreviations

CFU:

colony-forming unit

IC50:

inhibition of 50% growth

LED:

light-emitting diode

MB:

methylene blue

MHB:

Mullen-Hinton broth

%T:

percentage of transmittance

PDT:

photodynamic therapy

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Authors and Affiliations

  1. Chemistry Department, State University of Maringá, Paraná, Brazil

    Lílian S. Peloi, Rafael R. S. Soares, Caruos E. G. Biondo, Vagner R. Souza & Noboru Hioka

  2. Department of Pharmacy and Pharmacology, State University of Maringá, Paraná, Brazil

    Elza Kimura

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  1. Lílian S. Peloi
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  2. Rafael R. S. Soares
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  3. Caruos E. G. Biondo
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  4. Vagner R. Souza
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  5. Noboru Hioka
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  6. Elza Kimura
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Correspondence to Elza Kimura.

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Peloi, L.S., Soares, R.R.S., Biondo, C.E.G. et al. Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. J Biosci 33, 231–237 (2008). https://doi.org/10.1007/s12038-008-0040-9

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  • DOI: https://doi.org/10.1007/s12038-008-0040-9

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