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Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum

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Abstract

A RING zinc finger ankyrin protein gene, designated AdZFP1, was isolated from drought-tolerant Artemisia desertorum Spreng by mRNA differential display and RACE. Its cDNA was 1723 bp and encoded a putative protein of 445 amino acids with a predicted molecular mass of 47.9 kDa and an isoelectric point (pI) of 7.49. A typical C3HC4-type RING finger domain was found at the C-terminal region of the AdZFP1 protein, and several groups of ankyrin repeats were found at the N-terminal region. Alignments of amino acid sequence showed that AdZFP1 was 66% identical to the Arabidopsis thaliana putative RING zinc finger ankyrin protein AAN31869. Transcriptional analysis showed that AdZFP1 was inducible under drought stress in root, stem and leaf of the plant. Semi-quantitative reverse-transcriptase-polymerase chain reaction (RT-PCR) analysis showed that the transcript of AdZFP1 was strongly induced by exogenous abscisic acid (ABA) and also by salinity, cold and heat to some extent. Overexpression of the AdZFP1 gene in transgenic tobacco enhanced their tolerance to drought stress.

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Abbreviations

ABA:

abscisic acid

PEG:

polyethylene glycol

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse transcriptase-polymerase chain reaction

RWC:

relative water content

References

  • Bartels D and Nelson D E 1994 Approaches to improve stress tolerance using molecular genetics; Plant Cell Environ. 17 659–666

    Article  CAS  Google Scholar 

  • Bennett V and Chen L 2001 Ankyrins and cellular targeting of diverse membrane proteins to physiological sites; Curr. Opin. Cell. Biol. 13 61–67

    Article  CAS  Google Scholar 

  • Bohnert H J, Nelson D E and Jensen R G 1995 Adaptation to environmental stresses; Plant Cell 7 1099–1111

    Article  CAS  Google Scholar 

  • Borden K L B and Freemont P S 1996 The RING finger domain: a recent example of a sequence-structure family; Curr. Opin. Struct. Biol. 6 395–401

    Article  CAS  Google Scholar 

  • Borden K L, Boddy M N, Lally J, O’Reilly N J, Martin S, Howe K, Solomon E and Freemont P S 1995 The solution structure of the RING finger domain from the acute promyelocytic leukaemia proto-oncoprotein; PML EMBO J. 14 1532–1541

    Article  CAS  Google Scholar 

  • Bork P 1993 Hundreds of ankyrin-like repeats in functionally diverse proteins: mobile modules that cross phyla horizontally? Proteins 17 363–374

    Article  CAS  Google Scholar 

  • Bray E A 1993 Molecular responses to water deficit; Plant Physiol. 103 1035–1040

    Article  CAS  Google Scholar 

  • Bray E A1997 Plant responses to water deficit; Trends Plant Sci. 2 48–54

    Article  Google Scholar 

  • Chaves M M, Maroco J P and Pereira J S 2003 Understanding plant responses to drought from genes to the whole plant; Funct. Plant Biol. 30 239–264

    Article  CAS  Google Scholar 

  • Chen B J, Wang Y, Hu Y L, Wu Q and Lin Z P 2005 Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia; Plant Sci. 168 493–500

    Article  CAS  Google Scholar 

  • Chen W, Provart N J, Glazebrook J, Katagiri F, Chang H S, Eulgem T, Mauch F, Luan S, et al 2002 Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stress; Plant Cell 14 559–574

    Article  CAS  Google Scholar 

  • Freemont P S 1993 The RING finger: a novel protein sequence motif related to the zinc finger; Ann. N Y Acad. Sci. 684 174–192

    Article  CAS  Google Scholar 

  • Freemont P S 2000 Ubiquitination: RING for destruction?; Curr. Biol. 10 R84–R87

    Article  CAS  Google Scholar 

  • Freemont P S, Hanson I M and Trowsdale J 1991 A novel cysteine-rich sequence motif; Cell 64 483–484

    Article  CAS  Google Scholar 

  • Horsch R B, Fry J E, Hoffman N C, Eicholtz D, Rogers S G and Fraley R T 1985 A simple and general method for transforming gene into plants; Science 227 1229–1231

    Article  CAS  Google Scholar 

  • Jackson P K, Eldridge A G, Freed E, Furstenthal L, Hsu J Y, Kaiser B K and Reimann J D R 2000 The lore of the RINGs: substrate recognition and catalysis by ubiquitin ligases; Trends Cell Biol. 10 429–439

    Article  CAS  Google Scholar 

  • Joazeiro C A P and Weissman A M 2000 RING finger proteins: mediators of ubiquitin ligase activity; Cell 102 549–552

    Article  CAS  Google Scholar 

  • Laity J H, Lee B M and Wright P E 2001 Zinc finger proteins: new insights into structural and functional diversity; Curr. Opin. Struct. Biol. 11 39–46

    Article  CAS  Google Scholar 

  • Lee H, Xiong L, Gong Z, Ishitani M, Stevenson B and Zhu J K 2001 The Arabidopsis HOS1 gene negatively regulates cold signal transduction and encodes a RING finger protein that displays cold-regulated nucleo-cytoplasmic partitioning; Genes Dev. 15 912–924

    Article  CAS  Google Scholar 

  • Leung J and Girandat J 1998 Abscisic acid signal transduction; Annu. Rev. Plant Physiol. Plant Mol. Biol. 49 199–222

    Article  CAS  Google Scholar 

  • Liu N, Gao Y B, Jia C X and Ren A Z 2000 [Changes in POD activity, free praline content and cytomembrane permeability of Lolium multiflorum leaves under different levels of osmotic stress;] Plant Physiol. Commun. 36 11–14 (in Chinese)

    CAS  Google Scholar 

  • Lorick K L, Jensen J P, Fang S, Ong A M, Hatakeyama S and Weissman A M 1999 RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination; Proc. Natl. Acad. Sci. USA 96 11364–11369

    Article  CAS  Google Scholar 

  • McNellis T W, Arnim A G and Deng X W 1994 Overexpression of Arabidopsis COP1 results in partial suppression of light-mediated development: evidence for a light-inactivable repressor of photomorphogenesis; Plant Cell 6 1391–1400

    Article  CAS  Google Scholar 

  • McNellis T W, Torii K U and Deng X W 1996 Expression of an N-terminal fragment of COP1 confers a dominant-negative effect on light-regulated seedling development in Arabidopsis; Plant Cell 8 1491–1503

    Article  CAS  Google Scholar 

  • Rogers S R and Bendich A J 1994 Extraction of total cellular DNA from plants, algae and fungi; in Plant molecular biology manual (eds) S B Gelvin and R A Schilperoort (Dordrecht, The Netherlands: Kluwer Academic Publishers) 2nd edition, pp 1–8

    Google Scholar 

  • Rubtsov A M and Lopina O D 2000 Ankyrins; FEBS Lett. 482 1–5

    Article  CAS  Google Scholar 

  • Sambrook J and Russell D W 2001 Molecular cloning: a laboratory manual, 3rd edition (New York: Cold Spring Harbor Laboratory Press)

    Google Scholar 

  • Satijn D P, Gunster M J, Van der Vlag J, Hamer K M, Schul W, Alkema M J, Saurin A P, Freemont P S, Van Driel R and Otte A P 1997 RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor; Mol. Cell. Biol. 17 4105–4111

    Article  CAS  Google Scholar 

  • Sedgwick S G and Smerdon S J 1999 The ankyrin repeat: a diversity of interactions on a common structural framework; Trends Biochem. Sci. 24 311–316

    Article  CAS  Google Scholar 

  • Seki M, Ishida J, Narusaka M, Fujita M, Nanjo T, Umezawa T, Kamiya A, Nakajima M, Enju A and Sakurai T 2002a Monitoring the expression pattern of ca. 7000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray; Funct. Integ. Genom. 2 282–291

    Article  CAS  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozakiy K and Sekiz M 2003 Regulatory network of gene expression in the drought and cold stress responses; Curr. Opin. Plant Biol. 6 410–417

    Article  CAS  Google Scholar 

  • Waterman H, Levkowitz G, Alroy I and Yarden Y 1999 The RING finger of c-Cb1 mediates desensitization of the epidermal growth factor receptor; J. Biol. Chem. 274 22151–22154

    Article  CAS  Google Scholar 

  • Xiong L M, Schumaker K S and Zhu J K 2002 Cell signaling during cold, drought, and salt stress; Plant Cell S165–S183

  • Yang Y, Fang S, Jensen J P, Weissman A M and Ashwell J D 2000 Ubiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli; Science 288 874–877

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Life Sciences, National Key Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, People’s Republic of China

    Xiuhong Yang, Chao Sun, Yuanlei Hu & Zhongping Lin

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  1. Xiuhong Yang
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  2. Chao Sun
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  3. Yuanlei Hu
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  4. Zhongping Lin
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Correspondence to Zhongping Lin.

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Yang, X., Sun, C., Hu, Y. et al. Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum . J Biosci 33, 103–112 (2008). https://doi.org/10.1007/s12038-008-0026-7

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  • DOI: https://doi.org/10.1007/s12038-008-0026-7

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