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The LncRNA RP11-279C4.1 Enhances the Malignant Behaviour of Glioma Cells and Glioma Stem-Like Cells by Regulating the miR-1273g-3p/CBX3 Axis

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Abstract

Glioma is the most common type of solid tumour affecting the central nervous system, and the survival rate of patients with glioma is low. However, the mechanism associated with glioma progression remains unclear. Growing evidence suggests that lncRNAs play essential roles in the initiation and progression of tumours, including gliomas. In the present study, we identified and verified the expression of the novel lncRNA RP11-279C4.1 by analyzing the TANRIC database and performing qRT-PCR assays, the results of which revealed its upregulation in glioma tissues and cell lines. The results of multiple functional experiments demonstrated that RP11-279C4.1 knockdown inhibited glioma malignant phenotypes, including cell proliferation, migration, invasion and cell self-renew ability in vitro. In addition, RP11-279C4.1 downregulation suppressed tumour growth in vivo. Mechanistically, RP11-279C4.1 induced CBX3 activation via competitively sponging miR-1273g-3p, and rescue assay results confirmed the importance of the RP11-279C4.1/miR-1273g-3p/CBX3 axis. Overall, the results of our present study demonstrated that RP11-279C4.1 functions as an oncogene that promotes tumour progression by modulating the miR-1273g-3p/CBX3 axis in glioma, suggesting that RP11-279C4.1 may be a novel therapeutic target for glioma.

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All data generated or analyzed during this study are included in this published article.

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Funding

This study was supported by the Graduate Research and Innovation Projects of Jiangsu Province (KYCX19_0114).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Medical School of Southeast University, # 87 Dingjiaqiao, Nanjing, 210009, China

    Faming Wang, Yao Luo, Qingyun Zhang & Liudi Yuan

  2. Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing, 210096, China

    Le Zhang, Yueling Zhang, Yingying Shao & Liudi Yuan

Authors
  1. Faming Wang
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  2. Le Zhang
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  3. Yao Luo
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Contributions

LY and FW designed and supervised the study. FW, LZ and YL performed the in vitro and in vivo experiments. FW and QZ prepared the figs. YZ collected and analyzed the data. FW, YL and YS wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Liudi Yuan.

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Ethics Approval and Consent to Participate

All procedures performed in this study were approved by the Research Ethics Committee of Southeast University (Nanjing, China) (20200701006, 01 July 2020).

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Not applicable.

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The authors declare no competing interests.

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Supplementary Information

Supplementary Figure S1

The relative expression of miR-1273g-3p in glioma tissues and cell lines. a qRT-PCR was performed to analysis the expression level of miR-1273g-3p in one normal cell line and glioma cell lines. b The relative miR-1273g-3p expression in differaent grades of glioma was examined by CGGA database. c The overall survival were created by analyzing Oncomine database and classifified according to high and low miR-1273g-3p expression. The data are expressed as the mean ± SD of triplicates of three independent experiments (*P < 0.05, **P < 0.01) (PNG 234 kb)

High Resolution Image (TIF 7003 kb)

Supplementary Figure S2

Overexpression of CBX3 partly rescued sh-RP11-279C4.1-induced inhibitory effects on proliferation of glioma. Colony formation assay was applied to evaluate the proliferation ability of U87 and U251 transfected with sh-NC, sh-RP11-279C4.1 and sh-RP11-279C4.1+pcDNA-CBX3. The data are expressed as the mean ± SD of triplicates of three independent experiments (*P < 0.05, **P < 0.01) (PNG 137 kb)

High Resolution Image (TIF 6769 kb)

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Wang, F., Zhang, L., Luo, Y. et al. The LncRNA RP11-279C4.1 Enhances the Malignant Behaviour of Glioma Cells and Glioma Stem-Like Cells by Regulating the miR-1273g-3p/CBX3 Axis. Mol Neurobiol 58, 3362–3373 (2021). https://doi.org/10.1007/s12035-021-02337-6

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