Abstract
A simple protein-assisted method was reported to synthesize pepsin-conjugated Ag2S nanocrystals in aqueous solution. The morphology, composition and structure of the products were characterized by transmission electron microscopy, high-resolution transmission electron microscopy, energy-dispersive spectroscopy, selected area electron diffraction and X-ray diffraction measurements. The results showed that as-prepared monoclinic Ag2S nanocrystals are worm-like nanochains in shape with sizes about 25 nm in diameter and up to hundreds of nanometres in length. The multiple coordinate bonds of pepsin molecules to the surface of Ag2S nanocrystals make as-prepared samples have good colloidal stability and biocompatibility as elucidated by Fourier transform infrared examination. Thermogravimetry–differential scanning calorimetry analysis indicated that the obtained products are inorganic–organic nanocomposites and there is strong interaction between Ag2S and pepsin. This interaction could result in the change of hydrophilic environment of pepsin and consequently intrinsic fluorescence of protein was quenched by Ag2S nanocrystals. Furthermore, the nanochains assembly of particle–particle and rod–rod oriented attachment was discussed to investigate the growth mechanism.
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Sapra S, Mayilo S, Klar T A, Rogach A L and Feldmann J 2007 Adv. Mater. 19 569
Pan D C, Wang X L, Zhou Z H, Chen W, Xu C L and Lu Y F 2009 Chem. Mater. 21 2489
Rai S and Bokatial L 2011 Bull. Mater. Sci. 34 227
Ghosh D, Mondal S, Ghosh S and Saha A 2012 J. Mater. Chem. 22 699
Priya R and Kanmani S 2010 Bull. Mater. Sci. 33 85
Wang H J, Yu X H, Cao Y, Zhou B and Wang C F 2012 J. Inorg. Biochem. 113 40
Wang Y, Tang Z, Tan S and Kotov N A 2005 Nano Lett. 5 243
Yang L, Yang H, Yang Z, Cao Y, Ma X, Lu Z and Zheng Z 2008 J. Phys. Chem. B 112 9795
Zhang X, Yang L, Jiang Y, Yu B B, Zou Y G, Fang Y, Hu J S and Wan L J 2013 Chem. Asian J. 8 2483
Qin D, Zhang L, He G and Liu Q 2011 Mater. Lett. 66 7
Mahdi M A, Hassan J J, Kasim S J, Ng S S and Hassan Z 2014 Bull. Mater. Sci. 37 337
Mann S 1993 Nature 365 499
Boskey L 1998 J. Cell. Biochem. 72 83
Aizenberg J 2004 Adv. Mater. 16 1295
Dickerson M B, Sandhage K H and Naik R R 2008 Chem. Rev. 108 4935
Briggs B D and Knecht M R 2012 Phys. Chem. Lett. 3 405
Jiang J, Yu R, Zhu J, Yi R, Qiu G, He Y and Liu X 2009 Mater. Chem. Phys. 115 502
Liu X L, Zhu Y J, Zhang Q, Li Z F and Yang B 2012 Mater. Res. Bull. 47 4263
Huang P, Bao L, Yang D, Gao G, Lin J, Li Z, Zhang C and Cui D 2012 Chem. Asian J. 6 1156
Lu Q, Gao F and Komarneni S 2006 Nanotechnol. 17 2574
Yang H Y, Zhao Y W, Zhang Z Y, Xiong H M and Yu S N 2013 Nanotechnol. 24 055706
Kong Y, Chen J, Gao F, Li W, Xu X, Pandoli O, Yang H, Ji J and Cui D 2010 Small 6 2367
Guo Y, Zhang J, Yang L, Wang H, Wang F and Zheng Z 2010 Chem. Commun. 46 3493
Cao Y, Wang H J, Gao C, Sun Y Y, Yang L, Wang B Q and Zhou J G 2011 J. Nanopart. Res. 13 2759
Liu X, Liu R, Tang Y, Zhang L, Hou X and Lv Y 2012 Analyst 137 1473
Gray J J 2004 Curr. Opin. Struct. Biol. 14 110
Treuel L, Eslahian K A, Docter D, Lang T, Zellner R, Nienhaus K, Nienhaus G U, Stauber R H and Maskos M 2014 Phys. Chem. Chem. Phys. 16 15053
Ghosh D, Mondal S, Roy C N and Saha A 2013 Phys. Chem. Chem. Phys. 15 20354
Pathan H M, Salunkhe P V, Sankapal B R and Lokhande C D 2011 Mater. Chem. Phys. 72 105
Xu Z, Bando Y, Wang W, Bai X and Golberg D 2010 ACS Nano 4 2515
Misra S K, Dybowska A, Berhanu D, Luoma S N and Valsami-Jones E 2012 Sci. Total Environ. 438 225
Wataha J C, Hanks C T and Craig R G 1993 J. Biomed. Mater. Res. 27 227
Xu C, Yuan Z, Kohler N, Kim J, Chung M A and Sun S 2009 J. Am. Chem. Soc. 131 15346
Chen J, Zhang T, Feng L, Zhang M, Zhang X, Su H and Cui D 2013 Mater. Lett. 96 224
Chen J, Kong Y, Ji J, Ruan J, Wang K, Gao F and Cui D 2012 Nanoscale 4 4455
Kato K, Kawachi Y and Nakamura H 2014 J. Asian Ceram. Soc. 2 33
Xue Z, Hu B, Dai S and Du Z 2012 Mater. Chem. Phys. 136 771
Kuang D, Xu A, Fang Y, Liu H, Frommen C and Fenske D 2003 Adv. Mater. 15 1747
Kamatari Y O, Dobson C M and Konno T 2003 Prot. Sci. 12 717
Liu B and Zeng H C 2005 Small 1 566
Priyam A, Chatterjee A, Das S K and Saha A 2005 Res. Chem. Intermed. 31 691
Chatterjee A, Priyam A, Ghosh D, Mondal S, Bhattacharya S C and Saha A 2012 J. Lumin. 132 545
Acknowledgements
This work was supported by the Key Project of Henan Province Higher Educational Science and Technology Program (15A150070) and the Key Nature Science Foundation of Pingdingshan University (PDSU-QNJJ-2013003). We also thank the referees for helpful comments.
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QIN, D., ZHANG, L., DU, X. et al. Fabrication of worm-like Ag2S nanocrystals under mediation of protein. Bull Mater Sci 38, 1665–1671 (2015). https://doi.org/10.1007/s12034-015-0973-1
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DOI: https://doi.org/10.1007/s12034-015-0973-1