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Paired Box 5-Induced LINC00467 Upregulation Promotes the Progression of Laryngeal Squamous Cell Cancer by Triggering the MicroRNA-4735-3p/TNF Alpha-Induced Protein 3 Pathway

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A Correction to this article was published on 31 October 2022

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Abstract

LINC00467 was reported as an oncogenic gene in different types of human cancers. In this study, we investigated the molecular mechanisms of LINC00467 in the tumorigenesis of laryngeal squamous cell cancer (LSCC). RT-qPCR was utilized to detect the mRNA expression of genes, and western blot assay was used to determine the protein levels of TNF alpha-induced protein 3 (TNFAIP3). The cell viability was detected by CCK-8 assay. Transwell assays were conducted to determine the cell migration and invasion of LSCC cells, and the cell cycle was assessed by flow cytometry. The association between paired box 5 (PAX5), LINC00467, miR-4735-3p, and TNFAIP3 was verified using ChIP, RNA pull-down, or luciferase reporter assays. In our study, we found that LINC00467 was upregulated in LSCC, and knockdown of LINC00467 suppressed cell viability and metastasis of LSCC. Besides, LINC00467 transcription could be activated by PAX5 in LSCC. Furthermore, LINC00467 acted as competitive endogenous RNA (ceRNA) for miR-4735-3p to accelerate LSCC progression. In the meantime, TNFAIP3 was identified as a downstream gene of miR-4735-3p. Finally, TNFAIP3 overexpression could overturn the effects of miR-4735-3p mimic on LSCC cellular activities. In conclusion, our results demonstrated that PAX5-induced LINC00467 facilitated LSCC progression by inhibiting miR-4735-3p to increase TNFAIP3 expression.

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Funding

This work was supported by the National Natural Science Foundation of Zhejiang province, China (Y2100578 and Y2090486), Medical and health research project of Zhejiang Province, China (2017RC011),  the Project of Medical and health science and technology of Hangzhou, China (B20220098), and the Project of Public welfare application research of Huzhou municipal science and Technology Bureau, China (2021GY44).

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  1. Yuan Li and Yong Wu have contributed equally to this research and should be considered as co-first author.

Authors and Affiliations

  1. Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China

    Yuan Li, Lili Dai, Honglin Wu, Chaohui Chen, Jiayu Ni, Enli Jin & Xuehua Zhou

  2. Department of Otorhinolaryngology, The Third People’s Hospital of Deqing, Deqing, China

    Yuan Li

  3. Department of Otorhinolaryngology, The First People’s Hospital of Tongxiang, Jiaxing, China

    Yong Wu

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  1. Yuan Li
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Li, Y., Wu, Y., Dai, L. et al. Paired Box 5-Induced LINC00467 Upregulation Promotes the Progression of Laryngeal Squamous Cell Cancer by Triggering the MicroRNA-4735-3p/TNF Alpha-Induced Protein 3 Pathway. Mol Biotechnol 65, 655–667 (2023). https://doi.org/10.1007/s12033-022-00564-3

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