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Microbial Degradation of Forensic Samples of Biological Origin: Potential Threat to Human DNA Typing

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Abstract

Forensic biology is a sub-discipline of biological science with an amalgam of other branches of science used in the criminal justice system. Any nucleated cell/tissue harbouring DNA, either live or dead, can be used as forensic exhibits, a source of investigation through DNA typing. These biological materials of human origin are rich source of proteins, carbohydrates, lipids, trace elements as well as water and, thus, provide a virtuous milieu for the growth of microbes. The obstinate microbial growth augments the degradation process and is amplified with the passage of time and improper storage of the biological materials. Degradation of these biological materials carriages a huge challenge in the downstream processes of forensic DNA typing technique, such as short tandem repeats (STR) DNA typing. Microbial degradation yields improper or no PCR amplification, heterozygous peak imbalance, DNA contamination from non-human sources, degradation of DNA by microbial by-products, etc. Consequently, the most precise STR DNA typing technique is nullified and definite opinion can be hardly given with degraded forensic exhibits. Thus, suitable precautionary measures should be taken for proper storage and processing of the biological exhibits to minimize their decaying process by micro-organisms.

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Fig. 1
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(Modified from Pishchany and Skaar [94])

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  1. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, 470001, India

    Hirak Ranjan Dash

  2. Department of Life Science, Laboratory of Environmental Microbiology and Ecology (LEnME), National Institute of Technology, Rourkela, Odisha, 769008, India

    Surajit Das

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Dash, H.R., Das, S. Microbial Degradation of Forensic Samples of Biological Origin: Potential Threat to Human DNA Typing. Mol Biotechnol 60, 141–153 (2018). https://doi.org/10.1007/s12033-017-0052-5

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