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Over-Expression of AtPAP1 Transcriptional Factor Enhances Phenolic Acid Production in Transgenic Roots of Leonurus sibiricus L. and Their Biological Activities

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Abstract

This study examines the production of five phenolic acids (chlorogenic acid, neochlorogenic acid, ferulic acid, caffeic acid and p-coumaric acid) following over-expression of AtPAP1 transcription factor by four transgenic root clones of Leonurus sibiricus after Agrobacterium rhizogenes transformation. The AtPAP1 expression level was estimated by quantitative real-time PCR. High levels of phenolic acids were found in the transgenic roots of L. sibiricus and were determined by high-performance liquid chromatography–mass spectrometry analysis. Additionally, transgenic roots showed antimicrobial potential and cytotoxic activity on glioma cells in IV grade. Our results suggest that L. sibiricus transformed roots with AtPAP1 gene over-expression may represent a potential source of phenolic acids.

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Authors and Affiliations

  1. Department of Biology and Pharmaceutical Botany, Medical University of Lodz, ul. Muszyńskiego 1, 90-151, Lodz, Poland

    Przemysław Sitarek, Halina Wysokińska & Ewa Skała

  2. Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Banacha 12/13, Lodz, Poland

    Tomasz Kowalczyk

  3. Center for Research in Biosciences and Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, 1749-024, Lisbon, Portugal

    Patricia Rijo & Catarina Garcia

  4. Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003, Lisbon, Portugal

    Patricia Rijo & Catarina Garcia

  5. Department of Pneumology and Allergy, 1st Chair of Internal Medicine, Medical University of Lodz, Lodz, Poland

    Adam J. Białas

  6. Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/13, Lodz, Poland

    Marzena Wielanek

  7. Laboratory of Medical Genetics, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland

    Monika Toma & Tomasz Śliwiński

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  1. Przemysław Sitarek
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  2. Tomasz Kowalczyk
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Correspondence to Przemysław Sitarek.

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Sitarek, P., Kowalczyk, T., Rijo, P. et al. Over-Expression of AtPAP1 Transcriptional Factor Enhances Phenolic Acid Production in Transgenic Roots of Leonurus sibiricus L. and Their Biological Activities. Mol Biotechnol 60, 74–82 (2018). https://doi.org/10.1007/s12033-017-0048-1

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