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Heterogeneous Expression and Functional Characterization of Cellulose-Degrading Enzymes from Aspergillus niger for Enzymatic Hydrolysis of Alkali Pretreated Bamboo Biomass

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Abstract

Enzymatic hydrolysis of cellulosic biomass has caught much attention because of modest reaction conditions and environment friendly conditions. To reduce the cost and to achieve good quantity of cellulases, a heterologous expression system is highly favored. In this study, cellulose-degrading enzymes, GH3 family β-glucosidase (BGL), GH7 family-related cellobiohydrolases (CBHs), and endoglucanase (EG) from a newly isolated Aspergillus niger BE-2 are highly expressed in Pichia pastoris GS115. The strain produced EG, CBHs, and BGL enzymatic concentration of 0.56, 0.11, and 22 IU/mL, respectively. Mode of actions of the recombinant enzymes for substrate specificity and end product analysis are verified and found specific for cellulose degradation. Bamboo biomass saccharification with A. niger cellulase released a high level of fermentable sugars. Hydrolysis parameters are optimized to obtain reducing sugars level of 3.18 g/L. To obtain reducing sugars from a cellulosic biomass, A. niger could be a good candidate for enzymes resource of cellulase to produce reducing sugars from a cellulosic biomass. This study also facilitates the development of highly efficient enzyme cocktails for the bioconversion of lignocellulosic biomass into monosaccharides and oligosaccharides.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31170067, 21303142), the research fund of Fujian Provincial Natural Science Foundation (Grant No: 2012J05029), and the National Basic Research Program of China (973 Program, Grant No: 2010CB732201).

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Authors and Affiliations

  1. School of Life Science, Xiamen University, Xiamen, 361005, People’s Republic of China

    Nasir Ali & Zhang Ting

  2. Collage of Energy Research, Xiamen University, Xiamen, 361005, People’s Republic of China

    Hailong Li, Yong Xue, Lihui Gan, Jian Liu & Minnan Long

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  1. Nasir Ali
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Correspondence to Jian Liu or Minnan Long.

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Ali, N., Ting, Z., Li, H. et al. Heterogeneous Expression and Functional Characterization of Cellulose-Degrading Enzymes from Aspergillus niger for Enzymatic Hydrolysis of Alkali Pretreated Bamboo Biomass. Mol Biotechnol 57, 859–867 (2015). https://doi.org/10.1007/s12033-015-9878-x

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