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The K-Segments of the Wheat Dehydrin DHN-5 are Essential for the Protection of Lactate Dehydrogenase and β-Glucosidase Activities In Vitro

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Abstract

The wheat dehydrin DHN-5 has been previously shown to exhibit heat protecting effect on enzymatic activities. In order to understand the molecular mechanism by which DHN-5 exerts its protective function, we performed an approach to dissect the functional domains of DHN-5 responsible for this feature. In two distinct enzymatic assays, we found that the truncated forms of DHN-5 containing only one K- or two K-segments are able to protect albeit to less extent than the wild type protein, lactate dehydrogenase and β-glucosidase against damage induced by various stresses in vitro. However, the YS- and Φ-segments alone have no protective effects on these enzymes. Therefore, our study provides the evidence that the protective function of DHN-5 seems to be directly linked to its K-segments which through their amphipatic α-helical structure, may act to prevent protein aggregation.

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Acknowledgments

This study was supported by grants from the Ministry of Higher Education and Scientific Research, Tunisia.

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Authors and Affiliations

  1. Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P “1177’’, 3018, Sfax, Tunisia

    Marwa Drira, Walid Saibi, Faïçal Brini, Khaled Masmoudi & Moez Hanin

  2. Laboratory of Biomass Valorization and Protein Production in Eucaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P “1177’’, 3018, Sfax, Tunisia

    Ali Gargouri

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  1. Marwa Drira
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  2. Walid Saibi
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  3. Faïçal Brini
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  4. Ali Gargouri
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  5. Khaled Masmoudi
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  6. Moez Hanin
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Correspondence to Moez Hanin.

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Drira, M., Saibi, W., Brini, F. et al. The K-Segments of the Wheat Dehydrin DHN-5 are Essential for the Protection of Lactate Dehydrogenase and β-Glucosidase Activities In Vitro. Mol Biotechnol 54, 643–650 (2013). https://doi.org/10.1007/s12033-012-9606-8

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