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Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha

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Abstract

Malonyl-CoA is an essential precursor for fatty acid biosynthesis that is generated from the carboxylation of acetyl-CoA. In this work, a gene coding for acetyl-CoA carboxylase (ACC) was isolated from an oleaginous fungus, Mucor rouxii. According to the amino acid sequence homology and the conserved structural organization of the biotin carboxylase, biotin carboxyl carrier protein, and carboxyl transferase domains, the cloned gene was characterized as a multi-domain ACC1 protein. Interestingly, a 40% increase in the total fatty acid content of the non-oleaginous yeast Hansenula polymorpha was achieved by overexpressing the M. rouxii ACC1. This result demonstrated a significant improvement in the production of fatty acids through genetic modification in this yeast strain.

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Acknowledgments

This work was funded by a grant (BT-B-02-NG-B5-4905) from National Science and Technology Development Agency, Thailand. Rawisara Ruenwai was supported by the Thailand Graduate Institute of Science and Technology. We thank Prof. Morakot Tanticharoen and Dr. Sansanalak Rachdawong for their helpful comments on this work.

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Authors and Affiliations

  1. School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok, 10150, Thailand

    Rawisara Ruenwai & Supapon Cheevadhanarak

  2. Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok, 10150, Thailand

    Supapon Cheevadhanarak

  3. Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), King Mongkut’s University of Technology Thonburi, Bangkhuntien, Bangkok, 10150, Thailand

    Kobkul Laoteng

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  1. Rawisara Ruenwai
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Correspondence to Kobkul Laoteng.

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Ruenwai, R., Cheevadhanarak, S. & Laoteng, K. Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha . Mol Biotechnol 42, 327–332 (2009). https://doi.org/10.1007/s12033-009-9155-y

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