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Analysis of Novel Nonviral Gene Transfer Systems for Gene Delivery to Cells of the Musculoskeletal System

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Abstract

The aim of the present study was to evaluate the efficacy of novel nonviral gene delivery systems in cells of musculoskeletal origin. Primary cultures of lapine skeletal muscle cells, lapine articular chondrocytes, human cells from fibrous dysplasia and cell lines established from human osteosarcoma (SAOS-2), chondrosarcoma (CS-1), murine skeletal myoblasts (L8) and fibroblasts (NIH 3T3) were transfected with the P. pyralis luc or the E. coli lacZ genes using Nanofectin 1 and 2, Superfect, JetPEI, GeneJammer, Effectene, TransPass D2, FuGENE 6, Lipofectamine 2000, Dreamfect, Metafectene, Escort III, and calcium phosphate. Maximal transfection efficiency in lapine skeletal muscle cells was of 60.8 ± 21.2% using Dreamfect, 38.9 ± 5.0% in articular chondrocytes using Gene Jammer, 5.2 ± 8.0% in human cells from fibrous dysplasia using Lipofectamine 2000, 12.7 ± 16.2% in SAOS-2 cells using FuGENE 6, 29.9 ± 3.5% in CS-1 cells using Lipofectamine 2000, 70.7 ± 8.6% in L8 cells using FuGENE 6, and 48.9 ± 13.0% in NIH 3T3 cells using Metafectene. When the cells were transfected with a human IGF-I gene, significant amounts of the IGF-I protein were secreted. These results indicate that relatively high levels of transfection can be achieved using novel nonviral gene transfer methods.

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Acknowledgments

We thank L. Weissbach and F. Hornicek for providing the CS-1 cells, V. Flockerzi for the NIH 3T3 cells and Y. Mehraein for providing the SAOS-2 cells. H. Madry and M. Cucchiarini have been supported by grants from the Deutsche Forschungsgemeinschaft (DFG MA 2363/1-3 and DFG CU 55/1-3).

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  1. Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Building 37, Kirrbergerstr. 1, 66421, Homburg, Germany

    Patrick Orth, Anja Weimer, Gunter Kaul, Dieter Kohn, Magali Cucchiarini & Henning Madry

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  1. Patrick Orth
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  2. Anja Weimer
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  3. Gunter Kaul
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  5. Magali Cucchiarini
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  6. Henning Madry
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Correspondence to Henning Madry.

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Orth, P., Weimer, A., Kaul, G. et al. Analysis of Novel Nonviral Gene Transfer Systems for Gene Delivery to Cells of the Musculoskeletal System. Mol Biotechnol 38, 137–144 (2008). https://doi.org/10.1007/s12033-007-0071-8

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  • DOI: https://doi.org/10.1007/s12033-007-0071-8

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