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In vitro cytotoxicity and anti-cancer drug release behavior of methionine-coated magnetite nanoparticles as carriers

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Abstract

A novel and specific drug delivery for in vitro cancer targeted are developed successfully by a simple one-step method. A CoFe2O4@Methionine core–shell nanoparticle was prepared by the reflux assay which amino acid in the surface makes ferrite biocompatible, enhances its chemical stability, and improves the drug-loading capacity. The synthesized nanoparticles were characterized using FTIR, TGA, XRD, SEM, TEM, and VSM which coating amino acid on the surface of CoFe2O4 was confirmed by XRD and TGA. The appearance of a new peak for C≡N confirms the formation of Letrozole-loaded carrier in the FTIR. The vibrating sample magnetometer of both bare CoFe2O4 and Methionine-coated CoFe2O4 nanoparticles exhibited room-temperature superparamagnetic behavior with a saturation value of 46 emu/g and 16.8 emu/g, respectively. The morphology and size of samples were characterized by SEM and TEM that the average size of the particle was around 28–29 nm. The loading of Letrozole and the effect of pH (5, 7.4) on the release behavior of the carrier was studied. The result of the drug release in pH is equal to 5 was about 88% which higher than pH is equal to 7.4. Also, the preparation had been evaluated for determining its cytotoxicity using MCF-7, MDA-MB-231, and MCF10A cells as an in vitro model, and the result vitro experiments showed that CoFe2O4@Methionine could significantly reduce cancer in cells model. These results demonstrate that core–shell nanoparticle was prepared is biocompatible and have potential use as drug delivery.

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Acknowledgements

The manuscript was initially announced as a preprint at research square on December 3rd, 2021.

Funding

This research received no external funding.

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Authors and Affiliations

  1. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Faten Eshrati Yeganeh

  2. Department of Microbiology, Noor Dahesh Institute of Higher Education, Meymeh, Iran

    Amir Eshrati Yeganeh

  3. Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mahdi Fatemizadeh

  4. Department of Chemistry, Iran University of Science and Technology, Tehran, Iran

    Bahareh Farasati Far

  5. GenLab Biosolutions Private Limited, Bangalore, Karnataka, India

    Sameer Quazi

  6. Department of Biomedical Sciences, School of Life Sciences, Anglia Ruskin University, Cambridge, UK

    Sameer Quazi

  7. School of Health Sciences, The University of Manchester, Manchester, UK

    Sameer Quazi

  8. Department of Pharmacy, Gomal University, Dikhan, Pakistan

    Muhammad Safdar

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  1. Faten Eshrati Yeganeh
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  2. Amir Eshrati Yeganeh
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Correspondence to Sameer Quazi.

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Eshrati Yeganeh, F., Eshrati Yeganeh, A., Fatemizadeh, M. et al. In vitro cytotoxicity and anti-cancer drug release behavior of methionine-coated magnetite nanoparticles as carriers. Med Oncol 39, 252 (2022). https://doi.org/10.1007/s12032-022-01838-1

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