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Kefir induces apoptosis and inhibits cell proliferation in human acute erythroleukemia

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Abstract

Acute erythroleukemia is an uncommon subtype of acute myeloid leukemia which has been considered to be a subtype of AML with a worse prognosis. Intensive chemotherapy is the first line of treatment. In recent years, the effect of kefir on some malignancies has been experimented. Kefir is a kind of beverage, which obtained by incubation of kefir grains with raw milk. Kefir grains are a symbiotic complex of different kinds of yeasts and bacteria, especially lactic acid bacteria which gather in a mostly carbohydrate matrix, named kefiran. We investigated the effect of kefir on acute erythroleukemia cell line (KG-1) and peripheral blood mononuclear cells (PBMCs). The cell line and PBMCs were treated with different doses of kefir and milk and incubated for three different times. We used Polymixin B to block the lipopolysaccharide and NaOH (1 mol/l) to neutralize the acidic media. Viability was detected by MTT assay. Apoptosis and necrosis were assessed by annexin–propidium iodide staining. Our results showed that kefir induced apoptosis and necrosis in KG-1 cell line. It was revealed that kefir decreased proliferation in erythroleukemia cell line. We did not observe a remarkable effect of kefir on PBMCs. Our study suggested that kefir may have potential to be an effective treatment for erythroleukemia.

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Acknowledgments

This study was conducted with financial support of Isfahan University of Medical Sciences (IRAN) with grant number 393766.

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Authors and Affiliations

  1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran

    Fatemeh Jalali, Mohammadreza Sharifi & Rasoul Salehi

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  1. Fatemeh Jalali
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  2. Mohammadreza Sharifi
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  3. Rasoul Salehi
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Correspondence to Mohammadreza Sharifi.

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This study was approved by the local ethics committee of Isfahan University of Medical Sciences (IRAN) and the studies have been approved by the appropriate institutional and/or national research ethics committee and have been performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Jalali, F., Sharifi, M. & Salehi, R. Kefir induces apoptosis and inhibits cell proliferation in human acute erythroleukemia. Med Oncol 33, 7 (2016). https://doi.org/10.1007/s12032-015-0722-8

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