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B-Myb regulates snail expression to promote epithelial-to-mesenchymal transition and invasion of breast cancer cell

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Abstract

Breast cancer is the leading cause of cancer death in women worldwide, which is closely related to metastasis. Recent studies argue that breast cancer cells that have undergone epithelial-to-mesenchymal transition (EMT) acquire aggressive malignant properties, but the molecular mechanisms underlying this transition are poorly understood. In this study, we found that siRNA-mediated attenuation of B-Myb expression restored E-cadherin expression and cell–cell junction formation in breast cancer cells, suppressing cell invasion, anchorage-independent growth, and tumor formation. In contrast, the forced B-Myb expression decreased the expression of the epithelial marker E-cadherin, but increased the mesenchymal markers in breast cancer cells. We found that B-Myb upregulated expression of the key EMT regulator snail and that it mediated EMT activation and cell invasion by B-Myb.

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References

  1. Acloque H, Adams MS, Fishwick K, et al. Epithelial–mesenchymal transitions: the importance of changing cell state in development and disease. J Clin Invest. 2009;119:1438–49.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Bao B, Wang Z, Ali S, et al. Over-expression of FoxM1 leads to epithelial–mesenchymal transition and cancer stem cell phenotype in pancreatic cancer cells. J Cell Biochem. 2011;112:2296–306.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Barbera MJ, Puig I, Dominguez D, et al. Regulation of Snail transcription during epithelial to mesenchymal transition of tumor cells. Oncogene. 2004;23:7345–54.

    Article  CAS  PubMed  Google Scholar 

  4. Cano A, Perez-Moreno MA, Rodrigo I, et al. The transcription factor snail controls epithelial–mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol. 2000;2:76–83.

    Article  CAS  PubMed  Google Scholar 

  5. Chen J, Ou-Yang X, Gao J, et al. Knockdown of ribonuclease inhibitor expression with siRNA in non-invasive bladder cancer cell line BIU-87 promotes growth and metastasis potentials. Mol Cell Biochem. 2011;349:83–95.

    Article  CAS  PubMed  Google Scholar 

  6. Li H, Chen X, Gao Y, et al. XBP1 induces snail expression to promote epithelial- to-mesenchymal transition and invasion of breast cancer cells. Cell Signal. 2014a.

  7. Li H, Song F, Chen X, et al. Bmi-1 regulates epithelial-to-mesenchymal transition to promote migration and invasion of breast cancer cells. Int J Clin Exp Pathol. 2014;7:3057–64.

    CAS  PubMed Central  PubMed  Google Scholar 

  8. Liao XH, Wang N, Liu LY, et al. MRTF-A and STAT3 synergistically promote breast cancer cell migration. Cell Signal. 2014;26:2370–80.

    Article  CAS  PubMed  Google Scholar 

  9. Lo HW, Hsu SC, Xia W, et al. Epidermal growth factor receptor cooperates with signal transducer and activator of transcription 3 to induce epithelial–mesenchymal transition in cancer cells via up-regulation of TWIST gene expression. Cancer Res. 2007;67:9066–76.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Montagner M, Enzo E, Forcato M, et al. SHARP1 suppresses breast cancer metastasis by promoting degradation of hypoxia-inducible factors. Nature. 2012;487:380–4.

    Article  CAS  PubMed  Google Scholar 

  11. Nieto MA. Epithelial–mesenchymal transitions in development and disease: old views and new perspectives. Int J Dev Biol. 2009;53:1541–7.

    Article  PubMed  Google Scholar 

  12. Pan X, Xiong D, Yao X, et al. Up-regulating ribonuclease inhibitor inhibited epithelial-to-mesenchymal transition and metastasis in murine melanoma cells. Int J Biochem Cell Biol. 2012;44:998–1008.

    Article  CAS  PubMed  Google Scholar 

  13. Qiao Y, Jiang X, Lee ST, et al. FOXQ1 regulates epithelial–mesenchymal transition in human cancers. Cancer Res. 2011;71:3076–86.

    Article  CAS  PubMed  Google Scholar 

  14. Rosano L, Spinella F, Di Castro V, et al. Endothelin-1 promotes epithelial-to-mesenchymal transition in human ovarian cancer cells. Cancer Res. 2005;65:11649–57.

    Article  CAS  PubMed  Google Scholar 

  15. Schmalhofer O, Brabletz S, Brabletz T. E-cadherin, beta-catenin, and ZEB1 in malignant progression of cancer. Cancer Metastasis Rev. 2009;28:151–66.

    Article  CAS  PubMed  Google Scholar 

  16. Thiery JP, Acloque H, Huang RY, et al. Epithelial–mesenchymal transitions in development and disease. Cell. 2009;139:871–90.

    Article  CAS  PubMed  Google Scholar 

  17. Yoshida J, Horiuchi A, Kikuchi N, et al. Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progression. Med Mol Morphol. 2009;42:82–91.

    Article  CAS  PubMed  Google Scholar 

  18. Yuan H, Kajiyama H, Ito S, et al. ALX1 induces snail expression to promote epithelial-to-mesenchymal transition and invasion of ovarian cancer cells. Cancer Res. 2013;73:1581–90.

    Article  CAS  PubMed  Google Scholar 

  19. Zhu J, Pan X, Zhang Z, et al. Downregulation of integrin-linked kinase inhibits epithelial-to-mesenchymal transition and metastasis in bladder cancer cells. Cell Signal. 2012;24:1323–32.

    Article  CAS  PubMed  Google Scholar 

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No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. Department of Oncological Surgery, Luqiao Hospital of Taizhou Enze Medical Group, Luqiao, Zhejiang, China

    Deyou Tao & Guobin Jiang

  2. Department of Gynecology, Taizhou Central Hospital of Taizhou Enze Medical Group, #999 Donghai Road, Taizhou City, 318000, Zhejiang Province, China

    Yihong Pan

  3. Department of Pathology, Taizhou Central Hospital of Taizhou Enze Medical Group, Taizhou, China

    Hongsheng Lu

  4. Department of Oncology, Hangzhou Cancer Hospital, The First People’s Hospital of Hangzhou, Hangzhou, China

    Song Zheng

  5. Department of Oncological Surgery, Taizhou Hospital of Taizhou Enze Medical Group, Linhai, China

    Hui Lin & Feilin Cao

Authors
  1. Deyou Tao
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  2. Yihong Pan
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  3. Guobin Jiang
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  4. Hongsheng Lu
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  5. Song Zheng
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  6. Hui Lin
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  7. Feilin Cao
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Correspondence to Yihong Pan.

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Tao, D., Pan, Y., Jiang, G. et al. B-Myb regulates snail expression to promote epithelial-to-mesenchymal transition and invasion of breast cancer cell. Med Oncol 32, 412 (2015). https://doi.org/10.1007/s12032-014-0412-y

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  • DOI: https://doi.org/10.1007/s12032-014-0412-y

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