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Genetic polymorphisms in hypoxia-inducible factor-1a gene and its association with HBV-related hepatocellular carcinoma in a Chinese population

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Abstract

Previous studies have demonstrated that hypoxia-inducible factor-1a (HIF-1a) may play a vital role in the pathogenesis of hepatocellular carcinoma (HCC). However, the relationship between HIF-1a polymorphisms and HCC has not been thoroughly investigated. The aim of this study is to determine whether HIF-1a polymorphisms are associated with HCC through a case–control study. Two polymorphisms in the HIF-1a gene (rs11549465 and rs115494657) were examined in 157 hepatitis B virus (HBV)-related HCC patients and 173 healthy controls using the polymerase chain reaction–restriction fragment length polymorphism method. DNA sequencing was used to validate genotype results. There were no significant differences in the genotype and allele frequencies of HIF-1a rs11549465 and rs115494657 polymorphisms between the HBV-related HCC patients and healthy controls. However, the data revealed that subjects with the CG haplotype have a higher susceptibility to HBV-related HCC [odds ratio (OR) = 2.327, 95 % confidence interval (CI) = 1.578–4.721, P = 0.008]. In contrast, the CA haplotype was associated with a significantly decreased risk of HBV-related HCC (OR = 0.416, 95 % CI = 0.172–0.910, P = 0.025). HIF-1a rs11549465 and rs115494657 polymorphisms appeared to be irrelevant to HBV-related HCC. However, the HIF-1a CG and CA haplotypes might be a risk factor and a protective marker, respectively, for HBV-related HCC in a Chinese population. Further investigations with a larger sample size may be required to validate the genetic effects of HIF-1a polymorphisms on HBV-related HCC.

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Acknowledgments

We thank Scribendi.com for its linguistic assistance during the preparation of this manuscript. This research was supported by National Natural Science Foundation of China (No. 81260302) and Youth Science Foundation of Guangxi Medical University (GXMUYSF201334).

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Authors and Affiliations

  1. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

    Yanqiong Liu, Jingzhe Sui, Limin Zhai, Shi Yang, Li Huang, Cuiju Mo, Junrong Wu, Shan Li & Xue Qin

  2. Department of Clinical Laboratory, The Second People’s Hospital of Nanning, Nanning, Guangxi, China

    Liying Huang

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  1. Yanqiong Liu
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  2. Jingzhe Sui
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  3. Limin Zhai
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  4. Shi Yang
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  5. Li Huang
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  6. Liying Huang
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  7. Cuiju Mo
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  9. Shan Li
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Correspondence to Shan Li or Xue Qin.

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Yanqiong Liu and Jingzhe Sui have contributed equally to this work.

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Liu, Y., Sui, J., Zhai, L. et al. Genetic polymorphisms in hypoxia-inducible factor-1a gene and its association with HBV-related hepatocellular carcinoma in a Chinese population. Med Oncol 31, 200 (2014). https://doi.org/10.1007/s12032-014-0200-8

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