Abstract
MicroRNAs were reported to be involved in the modulation of tumor development. The aim of our study was to investigate the effect of miR-205 on proliferation and apoptosis of laryngeal squamous cell carcinoma (LSCC) and seek associations between miR-205 and Bcl-2 using in vitro and in vivo methods. Real-time qPCR was used to analyze the expression of miR-205 in LSCC samples and Hep-2 cell line. Apoptosis, cell cycle, and proliferation (MTT) assays were performed to test the apoptosis and proliferation of LSCC cells after miR-205 transfection. Bcl-2 expression in cells was assessed with Western blotting. The tumorigenicity of LSCC cells was evaluated in nude mice model. MiR-205 was significantly down-regulated in LSCC tissues compared to adjacent normal tissues. Lower expression of miR-205 was indicated to be statistically related with advanced clinical stage and T3–4 grades. We found that restoration of miR-205 down-regulated the proliferative markers of dihydrofolate reductase and proliferating cell nuclear antigen and apoptotic regulator of Bcl-2. The findings in vitro and in vivo showed miR-205 could suppress cell proliferation and induce cell apoptosis. In addition, Bcl-2 was identified as one of the direct targets of miR-205 in LSCC cells. These results suggest that miR-205 may play as a tumor suppressor in LSCC, probably by targeting Bcl-2 and serve as a potential target for therapeutic intervention.
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Acknowledgments
The research was supported by grants from the Heilongjiang Postdoctoral Fund (LBH-Z12157), the foundation of Heilongjiang Educational Committee (12531343), the foundation of Heilongjiang Health Bureau (2012-624), the Youth Foundation of the Second Affiliated Hospital of Harbin Medical University (QN2011-01), the National science Foundation of china (81241085, 81372902, 81272965), the key project of Natural Science Foundation of Heilongjiang Province of China (ZD201215/H1302), the Research Fund for the Doctoral Program of Higher Education of China (20102307110007), and the science and technology innovation talent research funds of Harbin (2012RFXXS072).
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The authors declare that they have no conflict of interest.
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Linli Tian and Jiarui Zhang are co-first authors. Ming Liu and Yanan Sun are co-corresponding authors.
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Tian, L., Zhang, J., Ge, J. et al. MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma. Med Oncol 31, 785 (2014). https://doi.org/10.1007/s12032-013-0785-3
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DOI: https://doi.org/10.1007/s12032-013-0785-3