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Up-regulation of tripartite motif-containing 29 promotes cancer cell proliferation and predicts poor survival in colorectal cancer

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Abstract

Tripartite motif-containing 29 (TRIM29), also known as ataxia-telangiectasia group D, is structurally a member of the tripartite motif family of proteins, which characterized by the conserved RING finger, B-box, and coiled-coil domains. TRIM29 functions as an oncogene or a tumor suppressor depending on the tumor types. In this study, we aim to evaluate whether TRIM29 affects the tumorigenesis and progression of colorectal cancer. The expression of TRIM29 was investigated using real-time PCR in 40 pairs of colorectal cancer tissues and immunohistochemistry on a tissue microarray containing 203 cases of primary colorectal cancer paired with non-cancerous tissues. Down-regulation of TRIM29 was achieved by transient transfection in RKO cell lines, and the effects of TRIM29 on tumor proliferation were evaluated by MTT and plate colony formation assays. Results indicated that TRIM29 expression was much higher in colorectal cancer tissues and significantly associated with the depth of tumor invasion, lymph node metastasis, distant metastasis, histological differentiation, vascular invasion, ki-67 index, and advanced tumor stage. Patients with TRIM29-positive tumors had a higher recurrence rate and poorer survival than patients with TRIM29-negative tumors. In multivariate analyses, the TRIM29 expression was an independent factor for determining colorectal cancer prognosis after surgery. Moreover, down-regulation of TRIM29 inhibited tumor cell proliferation in vitro. In conclusion, TRIM29 plays an important role in promoting colorectal cancer progression. Our findings suggest that TRIM29 may serve as a novel biomarker for tumor recurrence and survival for colorectal cancer patients.

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Acknowledgments

The project was supported by the grants from National Natural Science Foundation of China (81072008, 81172328) and Medical engineering crossing project grant funded by Shanghai Jiaotong University (YG2011MS59).

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Authors and Affiliations

  1. Department of General Surgery, Shanghai Jiao Tong University Affiliated First People’s Hospital, 85 Wujin Road, Shanghai, 200080, People’s Republic of China

    Tao Jiang, Dong-wang Yan & Zhi-hai Peng

  2. Department of Pathology, Shanghai Jiao Tong University Affiliated First People’s Hospital, 85 Wujin Road, Shanghai, 200080, People’s Republic of China

    Hua-mei Tang & Su Lu

  3. Department of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, 804 South Shengli Road, Yinchuan, 750004, People’s Republic of China

    Yin-xue Yang

Authors
  1. Tao Jiang
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  2. Hua-mei Tang
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  3. Su Lu
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  4. Dong-wang Yan
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  5. Yin-xue Yang
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  6. Zhi-hai Peng
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Corresponding authors

Correspondence to Yin-xue Yang or Zhi-hai Peng.

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Tao Jiang and Huamei Tang have contributed equally to this work.

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Jiang, T., Tang, Hm., Lu, S. et al. Up-regulation of tripartite motif-containing 29 promotes cancer cell proliferation and predicts poor survival in colorectal cancer. Med Oncol 30, 715 (2013). https://doi.org/10.1007/s12032-013-0715-4

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  • DOI: https://doi.org/10.1007/s12032-013-0715-4

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