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Inverse correlation between Naa10p and MMP-9 expression and the combined prognostic value in breast cancer patients

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Abstract

To analyze the expression profiles of N-a-acetyltransferase 10 protein (Naa10p/ARD1) and matrix metalloproteinase 9 (MMP-9) in human breast cancer and evaluate their possible prognostic values in breast cancer patients. Quantitative RT-PCR was used to evaluate mRNA expression of Naa10p and MMP-9 in 55 cases of fresh breast cancer tissues, and immunohistochemistry was performed for detecting Naa10p and MMP-9 proteins on breast cancer with tissue microarray containing 80 specimens. Furthermore, Naa10p and MMP-9 were measured in 253 breast cancer tissues from patients with up to 15-year follow-up. Survival curves were generated using the Kaplan–Meier method. Multivariate analysis was performed by using the Cox proportional hazard regression model to assess the prognostic values of Naa10p and MMP-9. Both Naa10p and MMP-9 expression in breast cancer tissues were significantly higher than those in the matched non-cancerous tissues (p = 0.000 for both). There was an inverse correlation between Naa10p and MMP-9 expression at mRNA and protein levels (p = 0.000 for both). Patients with MMP-9-positive expression had a poorer overall survival (OS) and disease-free survival (DFS) than those with MMP-9-negative expression (p = 0.001 for both). However, patients with Naa10p-positive expression had better OS and DFS (p = 0.000 for both). In addition, Naa10p-positive/MMP-9-negative patients had the best OS and DFS (p = 0.000 for both). In multivariate survival analysis, TNM stage, Naa10p expression, MMP-9 expression, and combined expression status of Naa10p/MMP-9 were independent prognostic factors related to OS (p = 0.000, 0.007, 0.012, and 0.000, respectively) and DFS (p = 0.000, 0.002, 0.014, and 0.000, respectively).The expression level of Naa10p was inversely correlated with that of MMP-9 in human breast cancer samples. Combined analysis of Naa10p and MMP-9 had a significantly increased value for determining the prognosis of breast cancer patients.

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Acknowledgments

The authors thank Dr. Yun-tao Xie (Breast Center, Peking University Cancer Hospital & Institute) for providing fresh breast cancer samples. This study was supported by National 973 Program of China (2009CB521805) and National Natural Science Foundation of China (81071658).

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, 52 Fucheng Road, Haidian District, Beijing, 100142, China

    Yan Zeng, Caiyun Liu, Yuanyuan Li, Lin Meng, Jian Wu, Like Qu & Chengchao Shou

  2. Key Laboratory of Xinjiang Endemic and Ethnic Disease, Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang, 832000, China

    Yan Zeng

  3. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Center Laboratory, Peking University Cancer Hospital & Institute, Beijing, 100142, China

    Bin Dong

  4. Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Minimally Invasive Gastrointestinal Surgery, Peking University Cancer Hospital & Institute, Beijing, 100142, China

    Beihai Jiang

  5. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Breast Center, Peking University Cancer Hospital & Institute, Beijing, 100142, China

    Ye Xu

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  1. Yan Zeng
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  2. Caiyun Liu
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Correspondence to Chengchao Shou.

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Zeng, Y., Liu, C., Dong, B. et al. Inverse correlation between Naa10p and MMP-9 expression and the combined prognostic value in breast cancer patients. Med Oncol 30, 562 (2013). https://doi.org/10.1007/s12032-013-0562-3

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  • DOI: https://doi.org/10.1007/s12032-013-0562-3

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