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miR-22 is down-regulated in gastric cancer, and its overexpression inhibits cell migration and invasion via targeting transcription factor Sp1

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Abstract

Accumulating evidence has shown that microRNAs are involved in multiple processes in cancer development and progression. Recently, miR-22 has been identified as a tumor-suppressing microRNA in many human cancers. However, the specific function of miR-22 in gastric cancer is unclear at this point. In this study, we first measured miR-22 expression level in 30 pairs of gastric cancer and matched normal tissues, two normal and six gastric cancer cell lines by real-time quantitative RT-PCR. We found that the expression of miR-22 in gastric cancer tissues and cell lines was much lower than that in normal control, respectively. Transfection of miR-22 expression plasmid could significantly inhibit the cell migration and invasion in SGC-7901 and NCL-N87 gastric cancer cell lines. Moreover, we also showed that Sp1 was negatively regulated by miR-22 at the posttranscriptional level, via a specific target site within the 3′UTR by luciferase reporter assay. The expression of Sp1 was inversely correlated with miR-22 expression in gastric cancer tissues, and knockdown of Sp1 by siRNA inhibited cell malignant behaviors. Thus, our findings suggest that miR-22 acts as tumor suppressor by targeting the Sp1 gene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miR-22 in gastric cancer.

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Acknowledgments

This study is supported by National Natural Science Foundation of China (81101614 and 81171995).

Conflict of interest

The authors have no conflict of interest to disclose.

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Authors and Affiliations

  1. Department of Gastroenterology, Shenzhen Sixth People’s Hospital (Nanshan Hospital), Shenzhen, 518052, People’s Republic of China

    Mei-Mei Guo

  2. Department of Gastroenterology, Heilongjiang Provincial Hospital, Haerbin, 150036, People’s Republic of China

    Li-Hua Hu & Peng Chen

  3. Department of Medicine, Cancer Hospital of Xinjiang Medical University, Urumqi, 830000, People’s Republic of China

    Yong-Qiang Wang

  4. Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, No. 41 Youhao North Road, Urumqi, 830000, People’s Republic of China

    Yong-Qiang Wang, Jian-Guo Huang, Ning Lu, Jiang-Hong He & Cheng-Gong Liao

Authors
  1. Mei-Mei Guo
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  2. Li-Hua Hu
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  3. Yong-Qiang Wang
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  4. Peng Chen
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  6. Ning Lu
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  7. Jiang-Hong He
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  8. Cheng-Gong Liao
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Corresponding author

Correspondence to Cheng-Gong Liao.

Additional information

Mei-Mei Guo, Li-Hua Hu and Yong-Qiang Wang contributed equally to the work.

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Guo, MM., Hu, LH., Wang, YQ. et al. miR-22 is down-regulated in gastric cancer, and its overexpression inhibits cell migration and invasion via targeting transcription factor Sp1. Med Oncol 30, 542 (2013). https://doi.org/10.1007/s12032-013-0542-7

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  • DOI: https://doi.org/10.1007/s12032-013-0542-7

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