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miR-130b is an EMT-related microRNA that targets DICER1 for aggression in endometrial cancer

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Abstract

Endometrial cancer (EC) is the most common gynecologic malignancy, but the molecular events involved in the development and progression of EC remain unclear. Certain microRNAs (miRNAs) and DICER1 play important roles in cell motility and survival. This study investigated the role of miR-130b and DICER1 in EC. We profiled miR-130b and DICER1 expression in clinical samples explored its relationship with clinical parameters. A luciferase reporter assay assessed the miR-130b targeting potential of DICER1. We show both in vitro and in vivo that miR-130b overexpression along with DICER1 dysfunction leads to tumor aggression and miRNA synthesis abnormalities that are related to cancer hallmarks through DICER1–miRNAs axis modulation. We also identify the mechanism related to this potential tumor predisposing phenotype: miR-130b and loss of DICER1 induced abnormal expression of EMT-related genes, which constitutes a loop regulation of the miR-130b–DICER1–EMT axis.

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Acknowledgments

This study was supported by Grants from the National Natural Science Funds of China (Nos. 81072139 and 81172476).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Shanghai Jiao Tong University Affiliated International Peace Maternity and Child Health Hospital, Shanghai, China

    Bi-Lan Li, Wen Lu, Ting-ting Yang, Junjie Qu, Xiayu Hong & Xiao-ping Wan

  2. Department of Obstetrics and Gynecology, Shanghai First People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Cong Lu & Xiao-ping Wan

Authors
  1. Bi-Lan Li
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  2. Cong Lu
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  3. Wen Lu
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  4. Ting-ting Yang
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  5. Junjie Qu
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  6. Xiayu Hong
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  7. Xiao-ping Wan
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Correspondence to Xiao-ping Wan.

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Li, BL., Lu, C., Lu, W. et al. miR-130b is an EMT-related microRNA that targets DICER1 for aggression in endometrial cancer. Med Oncol 30, 484 (2013). https://doi.org/10.1007/s12032-013-0484-0

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