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Down-regulated KLF17 expression is associated with tumor invasion and poor prognosis in hepatocellular carcinoma

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Abstract

Although the role of Krüppel-like factor 17 (KLF17) in regulating epithelial–mesenchymal transition (EMT) has been explored in breast cancer, its influence on primary hepatocellular carcinoma (HCC) remains unclear. This study aims to investigate the expression status of KLF17 in hepatocellular carcinoma (HCC) and the correlation between KLF17 expression and metastatic potential of HCC. KLF17 expression in HCC and adjacent liver tissues was studied by real-time PCR and Western blot, and the relationship between KLF17 expression and the clinicopathological features of HCC was evaluated in 60 patients. By using RNA interference technique, the correlation of KLF17 expression and metastatic potential was investigated by down-regulating KLF17 expression in HepG2 cells, and the effects of KLF17 down-regulation on cell migration, and invasion were then analyzed. Furthermore, the correlation between KLF17 expression and the surgical outcomes of a cohort of HCC patients was analyzed. Reduced expression of KLF17 is associated with a short survival time in clinical patients (P = 0.034). Low KLF17 expression is related to tumor T stage (P = 0.045), tumor size (P = 0.027), lymph node stage (P = 0.030), M stage (P = 0.048), and portal vein tumor thrombosis significantly in HCC. Reduced expression of KLF17 promoted motility and invasion ability of HepG2 cells and changed the expression of E-cadherin, ZO-1, Snai1, and vimentin (genes are associated with EMT). Overall, these findings suggest a repressing role of KLF17 in tumor invasion and a new prognostic indicator in directing therapy. It deserves further exploration.

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Acknowledgments

We thank Professor Zhiyong Huang of the Department of Surgery (Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, China) for the providing the samples of patients with HCC.

Conflict of interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Down-regulated KLF17 expression is associated with tumor invasion and poor prognosis in hepatocellular carcinoma.”

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Authors and Affiliations

  1. Department of Gastroenterology Disease Research, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, 1095 Jiefang Avenue, 430030, Wuhan, HuBei, China

    Yue-Ling Deng, Yuan Li, Dan Zeng, Zhen-Zhen Zhou, De-An Tian & Mei Liu

  2. Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Fu-Yao Liu

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  1. Fu-Yao Liu
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  2. Yue-Ling Deng
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  3. Yuan Li
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  4. Dan Zeng
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  5. Zhen-Zhen Zhou
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  6. De-An Tian
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  7. Mei Liu
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Correspondence to De-An Tian or Mei Liu.

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Fu-Yao Liu and Yue-Ling Deng contributed equally to this work.

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Liu, FY., Deng, YL., Li, Y. et al. Down-regulated KLF17 expression is associated with tumor invasion and poor prognosis in hepatocellular carcinoma. Med Oncol 30, 425 (2013). https://doi.org/10.1007/s12032-012-0425-3

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  • DOI: https://doi.org/10.1007/s12032-012-0425-3

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