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Different expression of CD146 in human normal and osteosarcoma cell lines

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Abstract

The CD146 cell membrane adhesion molecule is highly expressed on the cell surface of several tumours. The level of its expression has been found to correlate directly with tumour progression and metastatic potential, thus establishing CD146 as an important candidate of tumour growth and metastasis. In order to characterize its expression in human osteosarcoma (OS) cell lines, we have examined the CD146 expression at protein and RNA levels in both normal and tumour osteoblast-like cell lines by several methods. Our results indicate that CD146 protein is expressed at low levels in normal osteoblast cells whereas it is highly expressed in all OS cell lines analysed, (SaOS, MG-63, U-2OS). Moreover, CD146 overexpression was partially reduced in shYY1 cells, where the Yin Yang 1 transcription factor, also found over-expressed in human OS cells, has been silenced.

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Acknowledgments

We thank Dr. De Nigris and Dr. Rienzo for providing cells and oligonucleotides.

Conflict of interest

None.

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Authors and Affiliations

  1. Department of General Pathology, Chair of Clinical Pathology, Excellence Research Centre on Cardiovascular Disease, U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], Regional Reference Laboratory of Transplant Immunology [LIT], Azienda Universitaria Policlinico (AOU), 1st School of Medicine, Second University of Naples, Piazza Miraglia 2, 80138, Naples, Italy

    Concetta Schiano, Vincenzo Grimaldi, Amelia Casamassimi & Claudio Napoli

  2. Fondazione Studio Diagnostica Nucleare (SDN), IRCCS, Via Gianturco, 80134, Naples, Italy

    Teresa Infante & Claudio Napoli

  3. Department of Biochemistry, 1st School of Medicine, Second University of Naples, Via L. De Crecchio 7, 80138, Naples, Italy

    Alessandra Esposito & Alfonso Giovane

Authors
  1. Concetta Schiano
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  2. Vincenzo Grimaldi
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  3. Amelia Casamassimi
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  4. Teresa Infante
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  5. Alessandra Esposito
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  6. Alfonso Giovane
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  7. Claudio Napoli
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Corresponding author

Correspondence to Vincenzo Grimaldi.

Additional information

Concetta Schiano and Vincenzo Grimaldi have contributed equally to this study.

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Schiano, C., Grimaldi, V., Casamassimi, A. et al. Different expression of CD146 in human normal and osteosarcoma cell lines. Med Oncol 29, 2998–3002 (2012). https://doi.org/10.1007/s12032-012-0158-3

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  • DOI: https://doi.org/10.1007/s12032-012-0158-3

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