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PTP1B expression contributes to gastric cancer progression

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Abstract

Protein tyrosine phosphatase 1B (PTP1B), a member of the superfamily of protein tyrosine phosphatases, has been implicated in cancer pathogenesis. However, the role of PTP1B in the development of gastric cancer is unclear. The purpose of this study was to clarify the expression pattern and role of PTP1B in the gastric cancer. The expression of PTP1B in gastric cancer tissues was determined by immunohistochemical staining. Cell growth assay, soft agar colony formation assay, and tumorigenicity assay were used for examining proliferation, colony formation, and in vivo tumorigenesis of gastric cancer cells. The total levels and phosphorylated levels of Akt, extracellular signal-regulated kinase (Erk1/2), focal adhesion kinase (FAK), and Src were examined by western blotting, respectively. PTP1B was overexpressed in gastric cancer tissues (65/80) and correlated with tumor metastasis and tumor-node-metastasis stage. Overexpression of PTP1B promoted the proliferation and in vivo tumorigenesis of MKN45 cells and also increased the phosphorylation levels of Akt, Erk1/2, and FAK and the activity of Src. These results were conformed by knockdown of PTP1B in MKN28 cells. Therefore, our study suggested that PTP1B expression might play an important role in the development of gastric cancer.

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Acknowledgments

This research was supported in part by the grant of Science and Technology Commission of Shanghai Municipality No. 07jc14041.

Conflict of interest

We declare that we have no conflicts of interest.

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, Yijishan Hospital, Wannan Medicial College, 241001, Wuhu, Anhui, People’s Republic of China

    Jinguo Wang, Pei Wu & Jian Wu

  2. Shanghai Key Laboratory of Diagnosis and Treatment for Gastrointestinal Cancer, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, 200025, Shanghai, People’s Republic of China

    Bingya Liu, Xuehua Chen, Liping Su & Zhenggang Zhu

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  1. Jinguo Wang
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  2. Bingya Liu
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  3. Xuehua Chen
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  5. Pei Wu
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  6. Jian Wu
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  7. Zhenggang Zhu
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Correspondence to Zhenggang Zhu.

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Wang, J., Liu, B., Chen, X. et al. PTP1B expression contributes to gastric cancer progression. Med Oncol 29, 948–956 (2012). https://doi.org/10.1007/s12032-011-9911-2

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  • DOI: https://doi.org/10.1007/s12032-011-9911-2

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