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Anti-Sp17 monoclonal antibody with antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity activities against human ovarian cancer cells

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Abstract

Sperm protein 17 (Sp17) is a cancer testis antigen that has been shown to be overexpressed in a variety of gynecologic malignancies, in particular ovarian cancer. Emerging evidences indicate that Sp17 is involved in tumorigenesis and in the migration of malignant cells. It has been proposed as a useful target for tumor-vaccine strategies and a novel marker to define tumor subsets and predict drug response. However, the antitumor activity of anti-Sp17 monoclonal antibody (anti-Sp17 mAb) has not been investigated. In this study, the in vitro cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) activities of anti-Sp17 mAb were evaluated using Sp17-positive ovarian cancer cells as targets, Sp17-negative ovarian cancer cells as the control, and healthy human peripheral blood monocytes and healthy human serum as effectors. Our preliminary results indicate that the direct cytotoxicity of anti-Sp17 mAb against the investigated ovarian cancer cells was very weak. However, the cytotoxicity of anti-Sp17 mAb, mediated by peripheral blood mononuclear cells (PBMCs), as ADCC, or by human serum, as CDC, was relatively strong in the Sp17-positive ovarian cancer cells. This finding suggested that anti-Sp17 mAb could be a useful tool against ovarian cancer and may provide insight into the development of low side-effect targeting therapy for this malignant disease.

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Abbreviations

Sp17:

Sperm protein 17

Anti-Sp17 mAb:

Anti-Sp17 monoclonal antibody

ADCC:

Antibody-dependent cell-mediated cytotoxicity activity

CDC:

Complement-dependent cytotoxicity

CTA:

Cancer testis antigen

CTL:

Cytotoxic T lymphocytes

PBMCs:

Peripheral blood monocytes

mCRPs:

Membrane complement regulatory proteins

MAC:

Membrane attack complex

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Acknowledgments

The authors appreciate Liu Qi, PhD for the gift of SKOV3 and COC2 human ovary carcinoma cell lines. Funding support: This work was supported by a grant from National Nature Science Foundation of China (No. 81101978).

Conflict of interests

The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Institute of Medical Laboratory Sciences, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, People’s Republic of China

    Jia-xi Song, Wang-li Cao, Fang-qiu Li, Li-ning Shi & Xuan Jia

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  1. Jia-xi Song
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  2. Wang-li Cao
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Correspondence to Fang-qiu Li.

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Song, Jx., Cao, Wl., Li, Fq. et al. Anti-Sp17 monoclonal antibody with antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity activities against human ovarian cancer cells. Med Oncol 29, 2923–2931 (2012). https://doi.org/10.1007/s12032-011-0137-0

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  • DOI: https://doi.org/10.1007/s12032-011-0137-0

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