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MicroRNA-21 is involved in osteosarcoma cell invasion and migration

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Abstract

MicroRNAs are involved in different cancer-related processes. MicroRNA-21 (miR-21), as an oncomiR, is overexpressed in all kinds of tumors and the role of miR-21 in carcinogenesis is elucidated in many cancers gradually. However, the function of miR-21 in osteosarcoma is still unclear. In our study, we found that miR-21 was significantly overexpressed in osteosarcoma tissues. More importantly, we confirmed that knockdown of miR-21 greatly decreased cell invasion and migration of MG-63. Furthermore, we identified that RECK (reversion-inducing-cysteine-rich protein with kazal motifs), a tumor suppressor gene, was a direct target of miR-21. Finally, the expression of RECK protein negatively correlated with the expression of miR-21 in human osteosarcoma tissues, indicating the potential regulation of RECK by miR-21. Our results suggest that miR-21 expression has a key role in regulating cellular processes in osteosarcoma, likely through regulating RECK and may serve as a therapeutic target.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No: 30672130).

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Authors and Affiliations

  1. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Road 1277, 430022, Wuhan, China

    Wu Ziyan, Yang Shuhua & Liu Xiaoyun

  2. Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Weng Xiufang

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  1. Wu Ziyan
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  2. Yang Shuhua
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  3. Weng Xiufang
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  4. Liu Xiaoyun
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Correspondence to Liu Xiaoyun.

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Ziyan, W., Shuhua, Y., Xiufang, W. et al. MicroRNA-21 is involved in osteosarcoma cell invasion and migration. Med Oncol 28, 1469–1474 (2011). https://doi.org/10.1007/s12032-010-9563-7

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  • DOI: https://doi.org/10.1007/s12032-010-9563-7

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