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REG gamma: a potential marker in breast cancer and effect on cell cycle and proliferation of breast cancer cell

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Abstract

To investigate the expression and significance of proteasomes reactivator REG gamma (γ) in breast cancer. First, we showed the expression of REGγ in breast cancer, metastatic lymph nodes and normal breast tissues. Meanwhile, we also analyzed the relationship between REGγ and estrogen receptor (ER), CerBb-2, lymph nodes metastasis and clinical stage of breast cancer. REGγ expression was determined by immunohistochemical staining and western blot. Secondly, we detected the expression of REGγ and REGγ-mRNA in human breast cancer cell lines (MDA-MB-231, MCF-7) and human breast ductal epithelial cell line (HBL-100) by western blot and real-time PCR. Finally, in order to identify effect of REGγ on breast cancer cell cycle and proliferation, we constructed recombinant plasmid of PcDNA3.1-REGγ and designed siRNA for REGγ in vitro. Cell cycle was assayed by flow cytometer (FCM), proliferation was measured by methyl thiazolyl tetrazolium (MTT). The results demonstrated abnormal high expression of REGγ in breast cancer and its metastatic lymph nodes. REGγ expression was related to breast cancer and its status of ER, CerBb-2 and lymph nodes metastasis. REGγ is one of the potential markers in breast cancer. REGγ could facilitate the growth of breast cancer cells.

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Abbreviations

ER:

Estrogen receptor

PCNA:

Proliferating cell nuclear antigen

siRNA RNA:

Interfere

FCS:

Foetal calf serum

MTT:

Methyl thiazolyl tetrazolium

FCM:

Flow cytometer

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Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (No. 30670811). The authors thank Jean-Francois RODIER for his generous help of modified article. The authors thank Prof. Shen Zhen Zhou (Tumor Hospital of Shanghai, China) for his generous gift of MDA-MB-231 cell line.

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Authors and Affiliations

  1. Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, 400016, Chongqing, China

    Xiaoyi Wang, Jinxiang Tan, Tian Tian, Liang Ran & Guosheng Ren

  2. Department of Anaesthesiology, Affiliated Children’s Hospital, Chongqing Medical University, 400014, Chongqing, China

    Shengfen Tu

  3. Department of Oncological Surgery, Centre Paul Strauss, 67090, Strasbourg, France

    Jean-Francois Rodier

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  1. Xiaoyi Wang
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  2. Shengfen Tu
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  3. Jinxiang Tan
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  4. Tian Tian
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  5. Liang Ran
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  6. Jean-Francois Rodier
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  7. Guosheng Ren
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Correspondence to Guosheng Ren.

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Wang, X., Tu, S., Tan, J. et al. REG gamma: a potential marker in breast cancer and effect on cell cycle and proliferation of breast cancer cell. Med Oncol 28, 31–41 (2011). https://doi.org/10.1007/s12032-010-9546-8

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  • DOI: https://doi.org/10.1007/s12032-010-9546-8

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