Abstract
Cancer of the larynx is the commonest head and neck squamous cell carcinoma. The DJ-1 gene is a novel mitogen-dependent oncogene. Survivin is a structurally unique member of the inhibitors of apoptosis proteins. DJ-1 and survivin play important roles in carcinogenesis. The function of DJ-1, and the relationship between DJ-1 and survivin in laryngeal carcinoma, has never been explored. Small interfering RNAs (siRNAs) directed against the DJ-1 gene were initially transfected into laryngeal carcinoma Hep-2 cells with liposome. The viability of Hep-2 cells was then detected by the MTT assay. The changes in cell-cycle distribution were monitored by flow cytometry. Finally, changes in DJ-1 and survivin genes mRNA and protein levels were evaluated by semi-quantitative RT-PCR and Western blotting, respectively. Blocking expression of the DJ-1 gene with DJ-1-siRNA significantly suppressed the viability of Hep-2 cells. Treatment with DJ-1-siRNA resulted in a G2/M accumulation. Expressions of DJ-1 and survivin gene mRNA and protein levels were suppressed by DJ-1-siRNA in a dose-dependent manner. These data indicate, for the first time, that the DJ-1 gene may have an important role in the carcinogenesis of laryngeal carcinoma.
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Acknowledgments
This work was supported by grants from the Ningbo Social Developmental Key Research Project (grant number 2008C50019) and the K. C. Wong Magna Fund in Ningbo University.
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Shen, Z., Jiang, Z., Ye, D. et al. Growth inhibitory effects of DJ-1-small interfering RNA on laryngeal carcinoma Hep-2 cells. Med Oncol 28, 601–607 (2011). https://doi.org/10.1007/s12032-010-9474-7
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DOI: https://doi.org/10.1007/s12032-010-9474-7