Abstract
Solute carrier family 16 member 1 (SLC16A1) is a crucial transcription factor in modifying cancer progression and metastasis. However, its character in defining the clinical prognosis of human gliomas has not been illuminated. In our analysis from PREdiction of Clinical Outcomes from Genomic Profiles (PRECOG), The Cancer Genome Atlas (TCGA), and Chinese Glioma Genome Atlas (CGGA), we found that SLC16A1 mRNA expression level was significantly increased in high-grade gliomas in contrast to low-grade gliomas and non-tumor controls (P < 0.05). Kaplan–Meier analysis of four independent cohort studies from the Gene Expression Omnibus (GEO) profile, TCGA, and CGGA which consistently presented patients with high SLC16A1 mRNA expression displayed poor overall survival in high-grade glioma patients (P < 0.05 by log-rank test). Based on the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), the protein–protein interaction analysis of SLC16A1-regulated oncogenesis showed SLC16A1 as a potential hub protein. Immunohistochemical staining exhibited that SLC16A1 protein overexpressed in high-grade gliomas compared with low-grade clinical glioma samples. All these findings suggest that SLC16A1 expression has a positive correlation with WHO pathological grading and poor survival. SLC16A1 might be a potential biomarker of prognosis in human gliomas.
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The dataset used and/or analyzed during the current study are available from the corresponding author on a reasonable request.
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Acknowledgements
The authors acknowledge Chin Lin for statistical analyses.
Funding
This study was supported in part by grants from Taichung Armed Force General Hospital (108B03, TCAFGH-E-109049 to K.-C.H), the Ministry of Science and Technology (MOST 106–2314-B-016–012-MY3 and MOST 108–2314-B-016–026 -MY3 to D.-Y.H.), Tri-Service General Hospital (TSGH-C108-007–008-S05 and TSGH-C01-109016 to D.-Y.H), and Medical Affairs Bureau, Ministry of National Defense (MAB-106–019, MAB-107–009, MAB-108–022 and MAB-109–014 to D.-Y.H).
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Conceptualization: Hong-Han Lin, Dueng-Yuan Hueng and Kuang-Chen Hung; Data curation, Hong-Han Lin, Chia-Kuang Tsai, Ssu-Han Chen, Li-Chun Huang, Dueng-Yuan Hueng, and Kuang-Chen Hung. Formal analysis, Hong-Han Lin, Wen-Chiuan Tsai, Ssu-Han Chen, Li-Chun Huang, Dueng-Yuan Hueng, and Kuang-Chen Hung. Funding acquisition, Dueng-Yuan Hueng and Kuang-Chen Hung. Investigation, Hong-Han Lin, Chia-Kuang Tsai, Ssu-Han Chen, Dueng-Yuan Hueng, and Kuang-Chen Hung. Methodology, Hong-Han Lin, Wen-Chiuan Tsai, Ssu-Han Chen, Li-Chun Huang, Dueng-Yuan Hueng, and Kuang-Chen Hung. Project administration, Dueng-Yuan Hueng, and Kuang-Chen Hung. Resources, Wen-Chiuan Tsai and Kuang-Chen Hung. Software, Hong-Han Lin, and Wen-Chiuan Tsai. Supervision, Dueng-Yuan Hueng, and Kuang-Chen Hung, Validation, Hong-Han Lin, Chia-Kuang Tsai, Li-Chun Huang, Dueng-Yuan Hueng, and Kuang-Chen Hung. Writing—original draft, Hong-Han Lin. Writing—review & editing, Hong-Han Lin, Dueng-Yuan Hueng, and Kuang-Chen Hung.
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This study was approved by the ethics Committee of Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC (TSGHIRB No: B-102–10).
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Lin, HH., Tsai, WC., Tsai, CK. et al. Overexpression of Cell-Surface Marker SLC16A1 Shortened Survival in Human High-Grade Gliomas. J Mol Neurosci 71, 1614–1621 (2021). https://doi.org/10.1007/s12031-021-01806-w
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DOI: https://doi.org/10.1007/s12031-021-01806-w