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MicroRNA-107 Ameliorates Damage in a Cell Model of Alzheimer’s Disease by Mediating the FGF7/FGFR2/PI3K/Akt Pathway

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Abstract

Alzheimer’s disease (AD), the most prevalent representation of dementia, is a neurodegenerative disease resulting from the degenerative disturbance of the central nervous system. Previous studies have indicated that miR-107 is reduced in the brain neocortex of patients with AD; however, its underlying mechanism is not clear. Therefore, the objective of this study was to explore the question of whether miR-107 participates in AD development. The study confirmed that the miR-107 expression levels were dramatically decreased in patients with AD and in beta-amyloid (Aβ) (Aβ)-treated SH-SY5Y cells compared with control groups. Upregulation of miR-107 reversed the inhibitory role of Aβ on cell proliferation and viability. In addition, miR-107 upregulation also ameliorated the Aβ-induced inflammation and apoptosis of SH-SY5Y cells. Furthermore, using bioinformatic prediction, dual-luciferase reporter assay (DLRA), quantitative polymerase chain reaction (qPCR), and Western blot (WB), miR-107 was confirmed to reduce the expression level of FGF7, and it subsequently deactivated the FGFR2/PI3K/Akt pathway. Moreover, FGF7 overexpression counteracted the role of miR-107 in the viability, proliferation, inflammation, and apoptosis of Aβ-induced SH-SY5Y cells.

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Funding

This work was supported by the Project of National Natural Science Foundation of China (No. 81760847); the Key Research and Development Program of Science and Technology Plan Project of Guangxi (No. Guike AB16380324); the Key Project of National Natural Science Foundation of China (No. 2018GXNSFDA050018); the Project of Guangxi Key Laboratory of Chinese Medicine Foundation Research (No. 16-380-58-04); the Youth Innovation Research Team of Guangxi University of Traditional Chinese Medicine (No. 2016QT004); and the High-level Talent Team Cultivation Project of Qihuang Project of Guangxi University of Traditional Chinese Medicine (No. 2018003).

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Author notes

  1. Wei Chen and Lin Wu contributed equally to this work and should be considered as equal first coauthors.

Authors and Affiliations

  1. Department of Neurology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, China

    Wei Chen, Yueqiang Hu, Ni Liang, Hongling Qin & Nong Tang

  2. Guangxi Key Laboratory of Chinese Medicine Foundation Research, Guangxi University of Chinese Medicine, No. 89-9 Dongge Road, Nanning, 530023, Guangxi, China

    Wei Chen, Lin Wu, Yueqiang Hu & Nong Tang

  3. Scientific Laboratorial Centre Guangxi University of Chinese Medicine, Nanning, China

    Lin Wu

  4. Graduate College of Guangxi University of Traditional Chinese Medicine, Nanning, China

    Lingfei Jiang & Jing Chen

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  1. Wei Chen
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  2. Lin Wu
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Correspondence to Nong Tang.

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Chen, W., Wu, L., Hu, Y. et al. MicroRNA-107 Ameliorates Damage in a Cell Model of Alzheimer’s Disease by Mediating the FGF7/FGFR2/PI3K/Akt Pathway. J Mol Neurosci 70, 1589–1597 (2020). https://doi.org/10.1007/s12031-020-01600-0

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