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Determination of the Best Concentration of Streptozotocin to Create a Diabetic Brain Using Histological Techniques

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Abstract

Alzheimer’s disease (AD) is one of the most important disorders among neurodegenerative diseases which is characterized by neurofibrillary tangles and senile plagues. Intercerebroventricular (ICV) streptozotocin administration is a form of sAD which was applied to examine different factors following AD. Previous reports used different doses of streptozotocin (STZ) to create Alzheimer’s model, but no standard dose has been introduced. Therefore, we decided to investigate the best concentration of STZ to induce a diabetic brain with lowest mortality rate and high severity of destruction. We treated rats with three different doses of STZ (STZ 1.5, 2.25, and 3 mg/kg, ICV). Spatial memory for treated rats was evaluated by Morris water maze (MWM). Locomotor activities of rats were assessed by open field test. Histological observation such as immunohistochemistry, immunofluorescence, and Nissl staining were performed on the brain especially in CA1, CA3, and DG regions of hippocampal neurons at residues P-ser396 and P-ser404. Our data suggest that although the percentage hyperphosphorylation of tau protein by injection of STZ 3 mg/kg was about 10 % more than STZ 2.25 mg/kg compared to the control group, we considered the latter doses due to no effect on motor activities and enhance the number of glial cells.

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Acknowledgments

This work was supported by Tehran University and Institute Pasteur of Iran funds.

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Authors and Affiliations

  1. Laboratory of Neuro-Organic Chemistry, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran

    Marzieh Dehghan-Shasaltaneh & Gholam Hossein Riazi

  2. Laboratory of Systems Biology and Bioinformatics (LBB), Institute of Biochemistry and Biophysics, University of Tehran, Tehran, 1417614411, Iran

    Marzieh Dehghan-Shasaltaneh & Ali Masoudi-Nejad

  3. Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran

    Nasser Naghdi & Samira Choopani

  4. Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran

    Leila Alizadeh

  5. School of Advanced Medical Technologies, Iran University of Medical Sciences, Tehran, Iran

    Bahram Bolouri

Authors
  1. Marzieh Dehghan-Shasaltaneh
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  2. Nasser Naghdi
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  3. Samira Choopani
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  4. Leila Alizadeh
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  5. Bahram Bolouri
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  6. Ali Masoudi-Nejad
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  7. Gholam Hossein Riazi
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Correspondence to Gholam Hossein Riazi.

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These animals’ experiments were carried out in accordance with recommendations from the Declaration of Helsinki and the internationally accepted principles for the use of experimental animals. All efforts were made to minimize animal suffering.

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Dehghan-Shasaltaneh, M., Naghdi, N., Choopani, S. et al. Determination of the Best Concentration of Streptozotocin to Create a Diabetic Brain Using Histological Techniques. J Mol Neurosci 59, 24–35 (2016). https://doi.org/10.1007/s12031-015-0702-7

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  • DOI: https://doi.org/10.1007/s12031-015-0702-7

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