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Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells into Motorneuron-like Cells on Electrospun Gelatin Membrane

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Abstract

Human bone marrow-derived mesenchymal stem cells are potent types of cells with self renewal ability and immunomodulatory properties. They not only have the capacity to differentiate into mesodermal lineages, but they are also capable to transdifferentiate into neural cells in vitro and in vivo. From a biological point of view, the specification of cell fate in the central nervous system is largely dictated by retinoic acid and sonic hedgehog. In addition with inductive molecules, electrospun three dimensional (3D) scaffolds with similar properties to natural extracellular matrix represent a physiological environment that could better resemble the in vivo microenvironment in comparison with two dimensional culture systems. In this regard, the aim of this study was to examine whether induction of human BM-MSCs with retinoic acid (RA) and sonic hedgehog (Shh) in combination with electrospun gelatin scaffold could lead to better differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) into motorneuron-like cells in vitro.

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Acknowledgments

This study was financially supported by Iran National Science Foundation (INSF).

Conflicts of Interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

    Faezeh Faghihi & Mohammad Taghi Joghataei

  2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Esmaeil Mirzaei

  3. Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Arash Sarveazad & Mohammad Taghi Joghataei

  4. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Jafar Ai & Somayeh Ebrahimi Barough

  5. Brain and Spinal Injury Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Jafar Ai

  6. Department of Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

    Abolfazl Lotfi

  7. Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mohammad Taghi Joghataei

Authors
  1. Faezeh Faghihi
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  2. Esmaeil Mirzaei
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  3. Arash Sarveazad
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  4. Jafar Ai
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  5. Somayeh Ebrahimi Barough
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  6. Abolfazl Lotfi
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  7. Mohammad Taghi Joghataei
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Correspondence to Mohammad Taghi Joghataei.

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Faghihi, F., Mirzaei, E., Sarveazad, A. et al. Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells into Motorneuron-like Cells on Electrospun Gelatin Membrane. J Mol Neurosci 55, 845–853 (2015). https://doi.org/10.1007/s12031-014-0437-x

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  • DOI: https://doi.org/10.1007/s12031-014-0437-x

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