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Changes in the Basal Membrane of Dorsal Root Ganglia Schwann Cells Explain the Biphasic Pattern of the Peripheral Neuropathy in Streptozotocin-Induced Diabetic Rats

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Abstract

Peripheral neuropathy is one of the main complications of diabetes mellitus. The current study demonstrated the bimodal pattern of diabetic peripheral neuropathy found in the behavioral study of pain perception in parallel to the histopathological findings in dorsal root ganglia (DRGs) neurons and satellite Schwann cell basement membranes. A gradual decrease in heparan sulfate content, with a reciprocal increase in deposited laminin in the basement membranes of dorsal root ganglia Schwann cells, was shown in streptozotocin-treated rats. In addition, the characteristic biphasic pain profiles were demonstrated in diabetic rats, as shown by hypersensitivity at the third week and hyposensitivity at the tenth week post-streptozotocin injection, accompanied by a continuous decrease in the sciatic nerve conduction velocity. It appears that these basal membrane abnormalities in content of heparan sulfate and laminin, noticed in diabetic rats, may underline the primary damage in dorsal ganglion sensory neurons, simultaneously with the bimodal painful profile in diabetic peripheral neuropathy, simulating the scenario of filtration rate in diabetic kidney.

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Abbreviations

STZ:

Streptozotocin

DRG:

Dorsal root ganglion

IDDM:

Insulin-dependent diabetes mellitus

HSPGs:

Heparan sulfate proteoglycans

BMs:

Basement membranes

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Acknowledgments

Acknowledgments to Dr. Yoav Chapman (Department of Human Physiology and Pharmacology, Sackler Medical School, University of Tel Aviv) for his assistance and help in electrophysiological study, to Prof. Beka Solomon and Dr. Vered Lavie (Department of Human Microbiology and Biotechnology, J Wise Faculty of Life Science, University of Tel Aviv) for their assistance and help in immunostaining analysis.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Anatomy and Anthropology, Sackler Faculty of Medicine, University of Tel Aviv, Tel Aviv, 69978, Israel

    Maria Becker, Tali Benromano & Chaim G. Pick

  2. Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, University of Tel Aviv, Tel Aviv, Israel

    Maria Becker & Zvi Nevo

  3. NVR Labs, Ness Ziona, Israel

    Abraham Shahar

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  1. Maria Becker
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  2. Tali Benromano
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  3. Abraham Shahar
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  5. Chaim G. Pick
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Correspondence to Chaim G. Pick.

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Becker, M., Benromano, T., Shahar, A. et al. Changes in the Basal Membrane of Dorsal Root Ganglia Schwann Cells Explain the Biphasic Pattern of the Peripheral Neuropathy in Streptozotocin-Induced Diabetic Rats. J Mol Neurosci 54, 704–713 (2014). https://doi.org/10.1007/s12031-014-0424-2

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