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1H-MRS Assessment of the Therapeutic Effect of Bilateral Intraventricular BDNF Infusion into APP/PS1 Double Transgenic Mice

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Abstract

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by extracellular accumulation of amyloid deposits. Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor whose levels have been shown to be decreased in AD brains. BDNF supplementation can offer improvement in the course of AD. However, the means of assessment are still relatively limited. In the present study, 1H-MRS was used to evaluate the therapeutic effects of bilateral intraventricular BDNF infusion into Alzheimer’s disease APP/PS1 double transgenic mice. For comparison to the 1H-MRS observations, Fluoro-Jade B staining and immunofluorescence for beta amyloid peptides (Aβ), glial fibrillary acidic protein, and tropomyosin-related kinase B (TrkB) were also performed. Our results showed that N-acetylaspartate (NAA) levels increased and myoinositol levels decreased in the BDNF group compared with the PBS group. However, the BDNF group NAA level was still lower than the control group at 6 weeks after infusion. These changes correlated with increased immunoreactivity for TrkB, decreased compact Aβ peptide containing plaques, and decreased Fluoro-Jade B-positive cells in the BDNF-infused mice compared to vehicle controls. These findings demonstrate that 1H-MRS may be a promising means of evaluating the therapeutic effects of BDNF on AD.

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Acknowledgments

This research was supported by grants from National Natural Science Foundation of China (81171297; 81200934).

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Authors and Affiliations

  1. Department of Radiology, Tongji Hospital, Medical School of Tongji University, No. 389, Xincun Road, Putuo District, Shanghai, 200065, China

    Wei Zhang, Pei-jun Wang, Ming-hua Li, Guo-liang Wang, Ping Li & Xiao-long Gao

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  1. Wei Zhang
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  2. Pei-jun Wang
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  3. Ming-hua Li
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  4. Guo-liang Wang
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Correspondence to Pei-jun Wang.

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Zhang, W., Wang, Pj., Li, Mh. et al. 1H-MRS Assessment of the Therapeutic Effect of Bilateral Intraventricular BDNF Infusion into APP/PS1 Double Transgenic Mice. J Mol Neurosci 50, 434–442 (2013). https://doi.org/10.1007/s12031-013-9951-5

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  • DOI: https://doi.org/10.1007/s12031-013-9951-5

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