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HIF-1α Involves in Neuronal Apoptosis after Traumatic Brain Injury in Adult Rats

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Abstract

Hypoxia-inducible factor-1α (HIF-1α), a well-identified hypoxia-related protein, is involved in regulating the biological functions of various cell types including neurons. The traditional biological function of HIF-1α is promoting the transcription of some pro-survival genes when exposing to low oxygen conditions. Meanwhile, some studies also point out that HIF-1α shows the detrimental role in several central nervous system (CNS) disorders. Up to now, the knowledge of HIF-1α function in CNS is still limited. To investigate whether HIF-1α is involved in CNS impairment and repair, we employed a traumatic brain injury model in adult rats. Upregulation of HIF-1α was observed in the injured brain cortex by western blot analysis and immunohistochemistry staining. Terminal deoxynucleotidyl transferase deoxy-UTP nick-end labeling (TUNEL) and 4′,6-diamidino-2-phenylindole (DAPI) staining suggested that HIF-1α was relevant to neuronal apoptosis after brain injury. In addition, glutamate excitotoxic model of primary cortex neurons was introduced to further investigate the role of HIF-1α in neuronal apoptosis; the result implied HIF-1α was associated with the regulation of p53 and BNIP3 in the apoptotic neurons. Based on our data, we suggested that HIF-1α might play an important role in neuronal apoptosis after traumatic brain injury in rat, which might also provide a basis for the further study on its role in regulating the transcription of target genes in apoptotic neurons, and might gain a novel strategy for the clinical therapy for traumatic brain injury.

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Acknowledgements

The project was funded by the Open Science Foundation of the Jiangsu Province (KF200953).

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226002, People’s Republic of China

    Aihong Li, Yaohui Ni & Xin Chen

  2. Department of Pathogen Biology, Medical College of Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

    Xiaolei Sun

  3. Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226002, People’s Republic of China

    Aisong Guo

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  1. Aihong Li
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  2. Xiaolei Sun
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  3. Yaohui Ni
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  4. Xin Chen
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  5. Aisong Guo
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Correspondence to Aisong Guo.

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Li, A., Sun, X., Ni, Y. et al. HIF-1α Involves in Neuronal Apoptosis after Traumatic Brain Injury in Adult Rats. J Mol Neurosci 51, 1052–1062 (2013). https://doi.org/10.1007/s12031-013-0084-7

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  • DOI: https://doi.org/10.1007/s12031-013-0084-7

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