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The SDF-1 3′A Genetic Variation of the Chemokine SDF-1α (CXCL12) in Parallel with its Increased Circulating Levels is Associated with Susceptibility to MS: A Study on Iranian Multiple Sclerosis Patients

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Abstract

Immune system-related factors are important in pathogenesis of multiple sclerosis. The CXC chemokine SDF-1α (CXCL12) is involved in the immune responses. Hence, the aim of this study was to investigate the association between serum levels of SDF-1α (CXCL12) and its gene polymorphisms at position +801 with multiple sclerosis. In this experimental study, blood samples were collected from 100 multiple sclerosis patients and 100 healthy controls on EDTA pre-coated tubes. DNA was extracted and DNA samples were analyzed for SDF-1α (CXCL12) polymorphisms using PCR–RLFP in patients and controls. The serum levels of SDF-1α (CXCL12) were measured by ELISA. Demographic data were also collected by a questionnaire which was designed specifically for this study. Our results showed a significant difference between the A/A, A/G, and G/G genotype and A and G alleles of polymorphisms at position +801 of SDF-1α (CXCL12). Our results also showed that serum levels of SDF-1α (CXCL12) were markedly higher in patients than healthy controls, but no association was observed between SDF-1α (CXCL12) polymorphism and its serum levels. The results of this study might suggest the serum levels of SDF-1α (CXCL12) and its polymorphism play an important role in pathogenesis of multiple sclerosis. It is also worth noting that these factors could probably use as pivotal biological markers in the diagnosis of MS.

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Acknowledgments

The authors of this article take this chance to thank all MS patients and healthy control individuals who voluntarily participated in this research project. This project was supported by a grant from Rafsanjan University of Medical Sciences.

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Authors and Affiliations

  1. Department of Neurology, Ali-ebn-Abitaleb Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Hossein Azin

  2. Department of Social Medicine, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Reza Vazirinejad

  3. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Behzad Nasiri Ahmadabadi, Hossein Khorramdelazad, Ebrahim Rezazadeh Zarandi, Mojgan Noroozi Karimabad & Gholamhossein Hassanshahi

  4. Department of Immunology and Hematology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Kazemi Arababadi

  5. Infectious and Tropical Disease Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Kazemi Arababadi

  6. Physiology, Pharmacology Research Center, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Ali Shamsizadeh

  7. Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Houshang Rafatpanah

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  1. Hossein Azin
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  2. Reza Vazirinejad
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  3. Behzad Nasiri Ahmadabadi
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  4. Hossein Khorramdelazad
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  5. Ebrahim Rezazadeh Zarandi
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  6. Mohammad Kazemi Arababadi
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  7. Mojgan Noroozi Karimabad
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  8. Ali Shamsizadeh
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  9. Houshang Rafatpanah
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  10. Gholamhossein Hassanshahi
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Correspondence to Gholamhossein Hassanshahi.

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Azin, H., Vazirinejad, R., Ahmadabadi, B.N. et al. The SDF-1 3′A Genetic Variation of the Chemokine SDF-1α (CXCL12) in Parallel with its Increased Circulating Levels is Associated with Susceptibility to MS: A Study on Iranian Multiple Sclerosis Patients. J Mol Neurosci 47, 431–436 (2012). https://doi.org/10.1007/s12031-011-9672-6

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  • DOI: https://doi.org/10.1007/s12031-011-9672-6

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