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Prognostic Value of Free DNA Quantification in Serum and Cerebrospinal Fluid in Glioma Patients

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Abstract

Unlike uniformly truncated DNA released from apoptotic nondiseased cells, free DNA released from dead tumor cells varies in size. Free DNA has been considered as a candidate biomarker for malignant tumors. We obtained serum samples from 70 patients with glioma and 22 healthy volunteers as control and cerebrospinal fluid (CSF) samples from 20 patients with glioma and eight nonneoplastic controls with hydrocephalus or arachnoid cyst and performed preoperative analysis of free DNA concentration and integrity by a quantitative polymerase chain reaction. With two primers sets amplifying short and long free DNA fragments (ALU115 and ALU247), free DNA integrity was determined by ratio of the concentration of ALU247 over ALU115 (ALU247/115). Our results indicate that free DNA integrity and the ratio of long fragments to short fragments may be a useful diagnostic assay for glioma. In summary, the CSF-free DNA concentration and integrity may serve as a new marker for the diagnosis of glioma.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81000775/H2006), the Nature Science Foundation of China Ministry of Health (2010-2-025), and the Nature Science Foundation of Jiangsu Colleges and Universities Grant (09KJB320011).

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Authors and Affiliations

  1. Department of Neurosurgery, Surgical Comprehensive Laboratory, Hospital Affiliated of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Wei Shi, Chenglin Lv, Jing Qi, Wei Zhao, Xiujie Wu, Rongrong Jing, Xinhua Wu, Shaoqing Ju & Jian Chen

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  1. Wei Shi
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  2. Chenglin Lv
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  3. Jing Qi
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  4. Wei Zhao
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  5. Xiujie Wu
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  6. Rongrong Jing
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  7. Xinhua Wu
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  8. Shaoqing Ju
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  9. Jian Chen
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Corresponding authors

Correspondence to Shaoqing Ju or Jian Chen.

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Wei Shi and Chenglin Lv contributed equally to this work.

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Shi, W., Lv, C., Qi, J. et al. Prognostic Value of Free DNA Quantification in Serum and Cerebrospinal Fluid in Glioma Patients. J Mol Neurosci 46, 470–475 (2012). https://doi.org/10.1007/s12031-011-9617-0

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  • DOI: https://doi.org/10.1007/s12031-011-9617-0

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