Abstract
Nicotinic acetylcholine receptor (nAChR) modulation of a number of parameters of synaptic signaling in the brain has been demonstrated. It is likely that effects of nicotine are due to its ability to modulate network excitability as a whole. A pre-requisite to understanding the effects of nicotine on network properties is the elucidation of functional receptors. We have examined the distribution of functional nAChRs in the dentate gyrus granule cells and the CA3 region of the mammalian hippocampus using calcium imaging from acute slices. Our results demonstrate the presence of functional nAChRs containing the α7 subunit (α7-nAChRs) on mossy fiber boutons, CA3 pyramidal cells, and on astrocytes. In addition, both CA3 interneurons and granule cells show nicotinic signals. Our study suggests that functional nicotinic receptors are widespread in their distribution and that calcium imaging might be an effective technique to examine locations of these receptors in the mammalian brain.
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Funding was provided by the National Institute for Drug Abuse (RO1 DA 10266), the National Institute of Deafness and Communication Disorders (RO1 DC 008855), and a Scientist Development Grant from the American Heart Association (GS).
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Proceedings of the XIII International Symposium on Cholinergic Mechanisms
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Grybko, M., Sharma, G. & Vijayaraghavan, S. Functional Distribution of Nicotinic Receptors in CA3 Region of the Hippocampus. J Mol Neurosci 40, 114–120 (2010). https://doi.org/10.1007/s12031-009-9266-8
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DOI: https://doi.org/10.1007/s12031-009-9266-8