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Involvement of CAPON and Nitric Oxide Synthases in Rat Muscle Regeneration After Peripheral Nerve Injury

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Abstract

Carboxy-terminal PDZ ligand of nNOS (CAPON) protein, as an adaptor, binds to nNOS via the PDZ domain helping regulate neuronal nitric oxide synthase (nNOS) activity at post-synaptic sites in neurons (Jaffrey et al., Neuron, 20, 115–124, 1998). Recently, it has been reported that CAPON is present in mouse muscle and may be involved in mouse muscle growth, injury, and repair possibly by regulating the stability, activity, or position of nNOS (Segalat et al., Experimental Cell Research, 302, 170–179, 2005). The present study was to explore the expression patterns and roles of CAPON as well as NOS in rat muscle regeneration after nerve injury. Normal Sprague–Dawley rats were subjected to right sciatic nerve crush injury. Walking track analysis, real time polymerase chain reaction, Western blotting, in situ hybridization, immunocytochemistry, and co-immunoprecipitation techniques were used. It revealed that CAPON mRNA increased, which peaked on days 1 and 28, whereas nNOS mRNA underwent a downregulation in the ipsilateral gastrocnemius muscles after sciatic nerve injury. Their proteins approximately paralleled the mRNA expression. CAPON and nNOS were identified in the activated satellite cells or myotubes and their in vivo interaction was verified. However, eNOS and iNOS proteins suffered an upregulation and were detected in activated satellite cells or myotubes. These data suggest that CAPON and all these three isoforms of NOS might be involved in muscle regeneration after nerve injury. Further study is necessary for a better understanding of the potential functional link between CAPON, NOS, and muscle regeneration, with possible application to therapy for skeletal muscle repair from nerve injury.

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Acknowledgments

This work is supported by Nature Science Foundation of China (30300099, 30770488), Jiangsu Province Natural Scientific Grants (BK2003035) and the Grant of the Jiangsu Province Key Lab of Neuroregeneration.

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Authors and Affiliations

  1. The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, China

    Mengling Chen, Chun Cheng, Meijuan Yan, Shuqiong Niu, Shangfeng Gao, Shuxian Shi, Haiou Liu, Yongwei Qin & Aiguo Shen

  2. Institute of Neurobiology, Fudan University, Shanghai, 200032, China

    Mengling Chen

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  1. Mengling Chen
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  2. Chun Cheng
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  3. Meijuan Yan
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  4. Shuqiong Niu
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  6. Shuxian Shi
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  7. Haiou Liu
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  9. Aiguo Shen
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Correspondence to Aiguo Shen.

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M. Chen and C. Cheng contribute equally to this work.

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Chen, M., Cheng, C., Yan, M. et al. Involvement of CAPON and Nitric Oxide Synthases in Rat Muscle Regeneration After Peripheral Nerve Injury. J Mol Neurosci 34, 89–100 (2008). https://doi.org/10.1007/s12031-007-9005-y

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  • DOI: https://doi.org/10.1007/s12031-007-9005-y

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