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DNMT3B −579 G>T Promoter Polymorphism and Risk of Gallbladder Carcinoma in North Indian Population

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Abstract

Aim

Carcinoma of gallbladder (GBC) is a relatively rare but highly fatal disease. The DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B) −579 G>T promoter polymorphism (rs1569686) influences gene function and has been associated with various malignancies. Present population-based case-control study was undertaken to examine the potential association of DNMT3B −579 G>T variation with GBC in North Indian population.

Methods

Genotypes and allelic frequencies of the DNMT3B −579 G>T polymorphism were determined for 212 GBC patients and 219 controls using PCR-RFLP. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated for the association of DNMT3B polymorphism with GBC. Analysis of potential transcription factor binding sites was also identified in the region harboring the polymorphism.

Results

The DNMT3B −579 G>T polymorphism was found to be non-significantly associated with an overall increased risk of GBC (OR = 1.10 and 1.56 for T/G and G/G genotypes, respectively, P trend = 0.227). The increased risk was predominant in both male and female cohorts and also non-significantly in GBC patients with gallstone status (OR = 1.44; P = 0.280, OR = 1.06; P = 0.804 and OR = 1.45; P = 0.143, respectively).

Conclusion

DNMT3B −579 G>T polymorphism may alter susceptibility to GBC although it may not play a major role in the pathoetiology of this disease in North Indian population.

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Acknowledgement

The study was supported by fellowship grants from Counsel of Scientific and Industrial Research (CSIR) and Department of Science and Technology (DST), Government of India.

Declaration of Competing Interests

The author(s) declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, 226014, India

    Kshitij Srivastava, Anvesha Srivastava & Balraj Mittal

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  1. Kshitij Srivastava
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  2. Anvesha Srivastava
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  3. Balraj Mittal
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Correspondence to Balraj Mittal.

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Srivastava, K., Srivastava, A. & Mittal, B. DNMT3B −579 G>T Promoter Polymorphism and Risk of Gallbladder Carcinoma in North Indian Population. J Gastrointest Canc 41, 248–253 (2010). https://doi.org/10.1007/s12029-010-9156-x

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  • DOI: https://doi.org/10.1007/s12029-010-9156-x

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