Abstract
The role of forensic histopathology in routine practice is to establish the cause of death in particular cases. This is achieved on the basis of microscopic analysis of representative cell and tissue samples taken from the major internal organs and from abnormal findings made at autopsy. A prerequisite of this is adherence to the quality standards set out for conventional histological/cytological staining and enzyme histochemical and immunohistochemical methods. The interpretation of histological findings is performed by taking into account macroscopic autopsy findings and information on previous history. Histological analysis may prompt postmortem biochemical and chemical–toxicological investigations. The results of histological analysis need to be classified by experts in the context of the available information and the need to withstand the scrutiny of other experts.
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Janssen W. Forensische Histologie. Lübeck: Schmidt-Römhild; 1977.
Cummings PM, Trelka DP, Springer KM. Atlas of forensic histopathology. Cambridge: Cambridge University Press; 2011.
Perper JA, Wecht CH. Microscopic diagnosis in forensic pathology. Springfield: Charles C. Thomas; 1980.
Pampin JB, Villadiego MS, editors. Practical manual of forensic histopathology. New York: Nova Science; 2012.
Oehmichen M, Auer RN, König HG. Forensic neuropathology and associated neurology. Berlin: Springer; 2009.
Lau G, Lai SH. Forensic histopathology. In: Tsokos M, editor. Forensic pathology reviews, vol. 5. Totowa: Humana Press; 2008. p. 239–65.
Ferrara SD, Bajanowski T, Cecchi R, Snenghi R, Case C, Viel G. Bio-medical guidelines and protocols: survey and future perspectives in Europe. Int J Leg Med. 2010;120:293–9.
Evers H, Heidorn F, Gruber C, Laszkowski G, Risse M, Dettmeyer R, Verhoff MA. Investigative strategy for the forensic detection of sperm traces. Forensic Sci Med Pathol. 2009;5:182–8.
Vandewoestyne M, van Hoofstat D, van Nieuwerburgh F, Deforce D. Automatic detection of spermatozoa for laser capture microdissection. Int J Leg Med. 2009;123:169–75.
Dettmeyer RB. Forensic histopathology. Berlin: Springer; 2011. p. 219–20.
Lunetta P, Modell JH. Macroscopical, microscopical, and laboratory findings in drowning victims. In: Tsokos M, editor. Forensic pathology reviews, vol. 3. Totowa: Humana Press; 2005. p. 3–77.
de la Grandmaison GL, Charlier P, Durigon M. Usefulness of systematic histological examination in routine forensic autopsy. J Forensic Sci. 2010;55:85–8.
Bernhardi FDC, Saldiva PHN, Mauad T. Histological examination has a major impact on macroscopic necropsy diagnoses. J Clin Pathol. 2005;58:1261–4.
Molina DK, Wood LE, Frost RE. Is routine histopathologic examination beneficial in all medicolegal autopsies? Am J Forensic Med Pathol. 2007;28:1–3.
Roulson J, Benbow EW, Hasleton PS. Discrepancies between clinical and autopsy diagnosis and the value of post mortem histology: a meta-analysis and review. Histopathology. 2005;47:551–9.
Langlois NE. The use of histology in 638 coronial post-mortem examinations of adults: an audit. Med Sci Law. 2006;46:310–20.
Zaitoun AM, Fernandez C. The value of histological examination in the audit of hospital autopsies: a quantitative approach. Pathology. 1998;30:100–4.
Dettmeyer RB. Forensic histopathology. Berlin: Springer; 2011. p. 213–6.
Falzi G, Henn R, Spann W. Über pulmonale Fettembolien nach Traumen mit verschieden langer Überlebenszeit. Münch Med Wschr. 1964;106:978.
Hausmann R, Pregler C, Schellmann B. The value of Lugol’s iodine staining technique for the identification of vaginal epithelial cells. Int J Leg Med. 1994;106:298–301.
Ritchie S, Waugh D. The pathology of Armanni–Ebstein-diabetic nephropathie. Am J Pathol. 1957;33:1035–57.
Raekallio J. Enzymes histochemically demonstrable in the earliest phase of wound healing. Nature. 1960;188:234–5.
Dürwald W. Gerichtliche Medizin. 3rd ed. Leipzig: J.A. Barth; 1987. p. 87.
Sinicina I, Pankratz H, Bise K, Metevossian E. Forensic aspects of post-mortem histological detection of amniotic fluid embolism. Int J Leg Med. 2009;124:55–62.
Yunginger JW, Nelson DR, Squillace DL, Jones RT, Holley KE, Hyma BA, et al. Laboratory investigations of deaths due to anaphylaxis. J Forensic Sci. 1991;36:857–65.
Thomsen H, Held H. Immunohistochemical detection of C5b-9 (m) in myocardium: an aid in distinguishing infarction-induced ischemic heart muscle necrosis from other forms of lethal myocardial injury. Forensic Sci Int. 1995;71:87–95.
Campobasso CP, Dell Erba AS, Addante A, Zotti F, Marzullo A, Colona MF. Sudden cardiac death and myocardial ischemia indicators. A comparative study of four immunohistochemical markers. Am J Forensic Med Pathol. 2008;29:154–61.
Welte TB, Bohnert M, Pollak S. Prevalence of rhabdomyolsis in drug deaths. Forensic Sci Int. 2004;139:21–5.
Büttner A, Rohrmoser K, Mall G, Penning R, Weis S. Widespread axonal damage in the brain of drug abusers as evidenced by accumulation of beta-amyloid precursor protein (beta-APP): an immunohistochemical investigation. Addiction. 2006;101:1339–46.
Dettmeyer R, Sperhake JP, Müller J, Madea B. Cytomegalovirus-induced pneumonia and myocarditis in three cases of suspected sudden infant death syndrome (SIDS): diagnosis by immunohistochemical techniques and molecular pathologic methods. Forensic Sci Int. 2008;174:229–33.
Dokhan DB, Kovatich AJ, Miettinen M. Nonenzymatic antigen retrieval in immunohistochemistry—comparison between different antigen retrieval modalities and proteolytic digestion. Appl Immunohistochem. 1993;1:149–55.
Pileri SA, Roncador G, Ceccarelli C, Piccioli M, Briskomatis A, Sabattini E, et al. Antigen retrieval techniques in immunohistochemistry: comparison of different methods. J Pathol. 1997;183:116–23.
von Wasielewski R, Werner M, Nolte M, Wilkens L, Georgii A. Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies. Histochemistry. 1994;102:165–72.
Werner M, Wasieleweski VR, Komminoth P. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem Cell Biol. 1996;105:253–60.
Armstrong EJ, Engelhart DA, Jenkins AJ, Balraj EK. Homicidal ethylene glycol intoxication: a report of a case. Am J Forensic Med Pathol. 2006;27:151–5.
Pomara C, Fiore C, D’Errico S, Riezzo I, Fineschi V. Calcium oxalate crystals in acute ethylene glycol poisoning: a confocal laser scanning microscope study in a fatal case. Clin Toxicol. 2008;46:322–4.
Takahashi S, Kanetake J, Kanawaku Y, Funayama M. Brain death with calcium oxalate deposition in the kidney: clue to diagnosis of ethylene glycol poisoning. Leg Med. 2008;10:43–5.
Wollersen H, Erdmann F, Risse M, Dettmeyer R. Accidental fatal ingestion of colchicine-containing leaves—toxicological and histological findings. Leg Med. 2009;11:S498–9.
Dettmeyer RB. Forensic histopathology. Berlin: Springer; 2011. p. 403–5.
Fineschi V, Turillazi E, Neri M, Pomara C, Riezzo I. Histological age determination of venous thrombosis: a neglected forensic task in fatal pulmonary thromboembolism. Forensic Sci Int. 2009;186:22–8.
Sandritter W, Thomas C, editors. Histopathologie. v: F.K. Schattauer; 1977.
Xiaophong Z, Xiaorui C, Jun H, Qisheng Q. The contrast of immunohistochemical studies of myocardial fibrinogen and myoglobin in early myocardial ischemia in rats. Leg Med. 2002;4:47–51.
Amberg R. Immunmorphologische Reaktionsmuster des Myokard bei kardialen und nicht-kardialen Perfusionsstörungen. In: Bratzke H, Schröter A, editors. Immunhistochemie in der Rechtsmedizin. Egelsbach: Hänsel-Hohenhausen; 1995. p. 101–16.
Fineschi V, Baroldi G, Silver MD. Pathology of the heart and sudden death in forensic medicine. Boca Raton: CRC; 2006.
Brunt EM, Janney CG, Bisceglie AM, Neuschwander-Tetri BA, Bacon RR. Nonalcoholic steatohepatitis: a proposal for grading and staging of the histological lesions. Am J Gastroenterol. 1999;94:2467–74.
Ferries JAJ, Thompson PJ. A histological assessment of the incidence of alcoholic cardiomyopathy in subjects with alcohol associated liver disease. Can Soc Forensic Sci. 1981;14:113–33.
Osawa M, Satoh F, Horiuchi H, Tian W, Kugota N, Hasegawa I. Postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents: evaluation of clinical laboratory parameters and immunohistochemistry in three cases. Leg Med. 2008;10:143–7.
Tsokos M. Immunohistochemical detection of sepsis-induced lung injury in human autopsy material. Leg Med. 2003;5:73–86.
Tsokos M, Fehlauer F. Post-mortem markers of sepsis: an immunohisto-chemical study using VLA-4 (CD49d/CD29) and ICAM-1 (CD54) for the detection of sepsis-induced lung injury. Int J Leg Med. 2001;114:291–4.
Tsokos M, Erbersdobler A. Pathology of peliosis. Forensic Sci Int. 2005;49:25–33.
Dettmeyer RB. Forensic histopathology. Berlin: Springer; 2011. p. 150–2.
Üzin I, Akyildiz E, Akif M. Histopathological differentiation of skin lesions caused by electrocution, flame burns and abrasion. Forensic Sci Int. 2008;178:157–61.
Karch SB. Karch’s pathology of drug abuse. 4th ed. Boca Raton: CRC Press; 2009. p. 523–33.
Wollersen H, Erdmann F, Risse M, Dettmeyer R. Oxalate-crystals in different tissues following intoxication with ethylene glycol: three case reports. Leg Med. 2009;11:S488–90.
Kakar P, Millar-Craig M, Kamaruddin H, Burn S, Loganathan S. Clozapine induced myocarditis: a rare but fatal complication. Int J Cardiol. 2006;112:e5–6.
Kilian JG, Kerr K, Lawrence C, Celermajer DS. Myocarditis and cardiomyopathy associated with clozapine. Lancet. 1999;354:1841–5.
Clevenger CV, August TF, Shaw LM. Colchicine poisoning: report of a fatal case with body fluid analysis by GC/MS and histopathologic examination of post-mortem tissues. J Anal Toxicol. 1991;15:151–4.
Iacobuzio-Donahue CA, Lee EL, Abraham SC, Yardley JH, Wu TT. Colchicine toxicity: distinct morphologic findings in gastrointestinal biopsies. Am J Surg Pathol. 2001;25:1067–73.
Sannohe S, Makino Y, Kita T, Kuroda N, Shinozuka T. Colchicine poisoning resulting from accidental ingestion of meadow saffron (Colchicum autumnale). J Forensic Sci. 2002;47:1391–6.
Magdalan J, Ostrowska A, Podhorska-Okolow M, Piotrowska A, Izykowska I, Nowak M, et al. Early morphological and functional alterations in canine hepatocytes due to a-amanitin, a major toxin of Amanita phalloides. Arch Toxicol. 2009;83:55–60.
Matschke J, Püschel K, Glatzel M. Ocular pathology in shaken baby syndrome and other forms of infantile non-accidental head injury. Int J Leg Med. 2009;123:189–97.
Rasten-Almqvist P, Eksborg S, Rajs J. Myocarditis and sudden infant death syndrome. APMIS. 2002;110:469–80.
Dettmeyer R, Baasner A, Schlamann M, Padosch SA, Haag C, Kandolf R, Madea B. Role of virus-induced myocardial affections in sudden infant death syndrome (SIDS): a prospective postmortem study. Pediatr Res. 2004;55:947–52.
Casali MB, Lazzaro A, Gentile G, Blandino A, Ronchi E, Zoja R. Forensic grading of myocarditis: an experimental contribution to the distinction between lethal myocarditis and incidental myocarditis. Forensic Sci Int. 2012;223:78–86.
Püschel K, Lockemann U, Dietrich M. Recurrent fatal outcome of malaria infections due to late diagnosis. Dtsch Ärztebl. 1998;95:2697–700.
Byard RW. Unexpected death due to infectious mononucleosis. J Forensic Sci. 2002;47:202–4.
Byard RW, Manton N, Tsokos M. Sarcoidosis and mechanism of unexpected death. J Forensic Sci. 2008;53:460–4.
Byard RW. Sudden death in Marfan syndrome. In: Tsokos M, editor. Forensic pathology reviews, vol. 4. Totowa: Humana Press; 2006. p. 93–106.
Dressler J, Hanisch U, Kuhlisch E, Geiger KD. Neuronal and glial apoptosis in human traumatic brain injury. Int J Leg Med. 2007;121:365–75.
Hausmann R. Timing of cortical contusions in human brain injury. Morphological parameters for a forensic wound-age estimation. In: Tsokos M, editor. Forensic pathology reviews, vol. 1. Totowa: Humana Press; 2004. p. 53–75.
Oehmichen M, Jakob S, Mann S, Saternus KS, Pedal I, Meissner C. Macrophage subsets in mechanical brain injury (MBI)—a contribution to timing of MBI based on immunohistochemical methods: a pilot study. Leg Med. 2009;11:118–24.
Ogata M, Tsuganezawa O. Neuron-specific enolase as an effective immunohistochemical marker for injured axons after fatal brain injury. Int J Leg Med. 1999;113:19–25.
Cecchi R. Estimation of wound age: looking into the future. Int J Leg Med. 2010;124:523–36.
Kondo T. Timing of skin wounds. Leg Med. 2007;9:109–14.
Betz P, Nerlich A, Wilske J, Tübel J, Penning R, Eisenmenger W. Analysis of the immunohistochemical localization of collagen type III and V for the time-estimation of human skin wounds. Int J Leg Med. 1993;105(6):329–32.
Betz P, Nerlich A, Wilske J, Tubel J, Penning R, Eisenmenger W. Immunohistochemical localization of collagen types I and VI in human skin wounds. Int J Leg Med. 1993;106:31–4.
DiMaio VJM, Dana SE. Handbook of forensic pathology. 2nd ed. Boca Raton: Taylor & Francis; 2007.
Byard RW, Gilbert JD, Terlet J. Death associated with volatile substance inhalation—histologic, scanning electron microscopic and energy dispersive X-ray spectral analysis of lung tissue. Forensic Sci Int. 2007;171:118–21.
Leestma JE. Forensic neuropathology. 2nd ed. Boca Raton: CRC Press; 2009.
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Dettmeyer, R.B. The role of histopathology in forensic practice: an overview. Forensic Sci Med Pathol 10, 401–412 (2014). https://doi.org/10.1007/s12024-014-9536-9
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DOI: https://doi.org/10.1007/s12024-014-9536-9