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Genetic polymorphisms of 17 short tandem repeat loci on Y chromosome in central Croatian population

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Abstract

In forensic casework, Y-chromosome short tandem repeat (STR) haplotyping is used in human identification, paternity testing and sexual assault cases where Y-STRs provide a male-specific DNA profile. The aim of this study was to describe the genetic structure of Y chromosome in a central Croatian population. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre “Ivan Vučetić”. A total of 220 unrelated healthy men from central Croatia were selected for the purpose of this study. Genomic DNA was extracted using a Chelex procedure from FTA® cards. Y-chromosomal STRs were determined using the AmpFISTR Yfiler PCR amplification kit. The haplotype frequencies were determined by direct counting and analyzed using Arlequin 3.1 and analysis of molecular variance calculated with the Y chromosome haplotype reference database online analysis tool. A total of 212 haplotypes were identified, 204 of which were unique. Total haplotype diversity was 0.993. Locus diversity varied from 0.325 for DYS392 to 0.786 for DYS385. Discrimination capacity was 92.7%. Allele frequencies diversity was 0.615. Intermediate alleles 17.2, 18.2 and 19.2 were found at DYS458 locus. A comparison with published data for the European minimal haplotype set showed the closest relationship to the Croatian capital of Zagreb and Bosnia and Herzegovina with significant genetic distance from Slovenia and Austria. The central Croatian population is now well characterized in terms of Y-chromosome STRs, thus providing a solid basis for further forensic and genetic epidemiology studies.

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Acknowledgments

This study was funded by the Ministry of Interior. The authors thank Sunčica Papić and Marija Valić for technical support.

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Authors and Affiliations

  1. Department of Biology and Fibers, Division of Biology, Forensic Science Centre “Ivan Vučetić”, General Police Directorate, Ministry of Interior, Ilica 335, 10000, Zagreb, Croatia

    Branka Gršković & Siniša Merkaš

  2. Forensic Science Centre “Ivan Vučetić”, General Police Directorate, Ministry of Interior, Ilica 335, 10000, Zagreb, Croatia

    Gordan Mršić & Andro Vrdoljak

  3. University Center for Forensic Sciences, University of Split, Ruđera Boškovića 31, 21000, Split, Croatia

    Gordan Mršić, Andro Vrdoljak & Šimun Anđelinović

  4. Department of Public Health, Medical School, University of Split, Šoltanska 2, 21000, Split, Croatia

    Ozren Polašek

  5. Department of Pathology and Forensic Medicine, Split University Hospital and School of Medicine, Spinčićeva 1, 21000, Split, Croatia

    Šimun Anđelinović

Authors
  1. Branka Gršković
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  2. Gordan Mršić
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  3. Ozren Polašek
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  4. Andro Vrdoljak
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  5. Siniša Merkaš
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  6. Šimun Anđelinović
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Correspondence to Branka Gršković.

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Gršković, B., Mršić, G., Polašek, O. et al. Genetic polymorphisms of 17 short tandem repeat loci on Y chromosome in central Croatian population. Forensic Sci Med Pathol 7, 155–161 (2011). https://doi.org/10.1007/s12024-010-9216-3

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  • DOI: https://doi.org/10.1007/s12024-010-9216-3

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