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Analysis of IMP3 Expression in Normal and Neoplastic Human Pituitary Tissues

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Abstract

Insulin-like growth factor II mRNA-binding protein 3 (IMP3) is an oncofetal protein highly expressed in fetal tissue and malignant tumors but rarely found in adult benign tissues. In various tumors, IMP3 expression is correlated with increased tumor aggressiveness and reduced overall survival. To our knowledge, IMP3 expression has not been investigated in pituitary tumors. We analyzed the immunohistochemical expression of IMP3 in five normal pituitary tissues and 75 pituitary tumors (64 adenomas and 11 carcinomas) to determine if specific tumor types expressed IMP3 and if there were differences in IMP3 expression between adenomas and carcinomas. Immunohistochemical analysis showed that IMP3 was positive in four (80%) normal pituitaries with focal stain in a subset of normal anterior pituitary cells. IMP3 was expressed in 31% (20/64) of adenomas and in 36% (4/11) of carcinomas. A slightly higher level of IMP3 expression was observed in PRL-GH-TSH adenomas compared to the other types of pituitary adenomas. Expression of IMP3 was not significantly higher in carcinomas than in adenomas (p = 0.737). RT-PCR and Western Blotting supported the heterogeneous expression of IMP3. These results indicate that IMP3 is expressed both in normal and in neoplastic pituitary gland tissues without significant differences in expression levels in pituitary carcinomas.

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Acknowledgements

This study was supported by the OTKA K68660 grant.

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Authors and Affiliations

  1. Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, 55905, USA

    Alberto Righi, Shuya Zhang, Long Jin, Bernd W. Scheithauer & Ricardo V. Lloyd

  2. Department of Biomedical Sciences and Oncology, University of Turin, Turin, Italy

    Alberto Righi

  3. Department of Laboratory Medicine, Division of Pathology, St. Michael’s Hospital, Toronto, ON, Canada

    Kalman Kovacs

  4. 2nd Department of Internal medicine, State Health Center, Budapest, Hungary

    Gabor Kovacs & Miklos I. Goth

  5. Department of Endocrinology, Barts and the London Medical School, Queen Mary University of London, London, UK

    Marta Korbonits

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  1. Alberto Righi
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  2. Shuya Zhang
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  3. Long Jin
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  4. Bernd W. Scheithauer
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  9. Ricardo V. Lloyd
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Correspondence to Ricardo V. Lloyd.

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Righi, A., Zhang, S., Jin, L. et al. Analysis of IMP3 Expression in Normal and Neoplastic Human Pituitary Tissues. Endocr Pathol 21, 25–31 (2010). https://doi.org/10.1007/s12022-009-9096-9

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  • DOI: https://doi.org/10.1007/s12022-009-9096-9

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