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Nonspecific binding of a frequently used vitamin D receptor (VDR) antibody: important implications for vitamin D research in human health

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References

  1. A.C. Ross, J.E. Manson, S.A. Abrams, J.F. Aloia, P.M. Brannon, S.K. Clinton, R.A. Durazo-Arvizu, J.C. Gallagher, R.L. Gallo, G. Jones, C.S. Kovacs, S.T. Mayne, C.J. Rosen, S.A. Shapses, The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know. J. Clin. Endocrinol. Metab. 96(1), 53–58 (2011). doi:10.1210/jc.2010-2704

    Article  CAS  PubMed  Google Scholar 

  2. H. Fuleihan Gel, R. Bouillon, B. Clarke, M. Chakhtoura, C. Cooper, M. McClung, R.J. Singh, Serum 25-hydroxyvitamin D levels: variability, knowledge gaps, and the concept of a desirable range. J. Bone Miner. Res. 30(7), 1119–1133 (2015). doi:10.1002/jbmr.2536

    Article  Google Scholar 

  3. C.J. Rosen, J.S. Adams, D.D. Bikle, D.M. Black, M.B. Demay, J.E. Manson, M.H. Murad, C.S. Kovacs, The nonskeletal effects of vitamin D: an Endocrine Society scientific statement. Endocr. Rev. 33(3), 456–492 (2012). doi:10.1210/er.2012-1000

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. L. Cianferotti, C. Cricelli, J.A. Kanis, R. Nuti, J.Y. Reginster, J.D. Ringe, R. Rizzoli, M.L. Brandi, The clinical use of vitamin D metabolites and their potential developments: a position statement from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) and the International Osteoporosis Foundation (IOF). Endocrine 50(1), 12–26 (2015). doi:10.1007/s12020-015-0606-x

    Article  CAS  PubMed  Google Scholar 

  5. S. Christakos, M. Hewison, D.G. Gardner, C.L. Wagner, I.N. Sergeev, E. Rutten, A.G. Pittas, R. Boland, L. Ferrucci, D.D. Bikle, Vitamin D: beyond bone. Ann. N. Y. Acad. Sci. 1287, 45–58 (2013). doi:10.1111/nyas.12129

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. D.D. Bikle, Vitamin D and cancer: the promise not yet fulfilled. Endocrine 46(1), 29–38 (2014). doi:10.1007/s12020-013-0146-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. R. Bouillon, L. Verlinden, Does a better vitamin D status help to reduce cardiovascular risks and events?. Endocrine 47(3), 662–663 (2014). doi:10.1007/s12020-014-0429-1

    Article  CAS  PubMed  Google Scholar 

  8. R. Bouillon, G. Carmeliet, L. Verlinden, E. van Etten, A. Verstuyf, H.F. Luderer, L. Lieben, C. Mathieu, M. Demay, Vitamin D and human health: lessons from vitamin D receptor null mice. Endocr. Rev. 29(6), 726–776 (2008). doi:10.1210/er.2008-0004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. S. Christakos, P. Dhawan, A. Verstuyf, L. Verlinden, G. Carmeliet, Vitamin D: metabolism, molecular mechanism of action, and pleiotropic effects. Physiol. Rev. 96(1), 365–408 (2016). doi:10.1152/physrev.00014.2015

    Article  PubMed  Google Scholar 

  10. C. Carlberg, Genome-wide (over)view on the actions of vitamin D. Front. Physiol. 5, 167 (2014). doi:10.3389/fphys.2014.00167

    PubMed  PubMed Central  Google Scholar 

  11. R.M. Evans, The nuclear receptor superfamily: a rosetta stone for physiology. Mol. Endocrinol. 19(6), 1429–1438 (2005). doi:10.1210/me.2005-0046

    Article  CAS  PubMed  Google Scholar 

  12. Y. Wang, B.R. Becklund, H.F. DeLuca, Identification of a highly specific and versatile vitamin D receptor antibody. Arch. Biochem. Biophys. 494(2), 166–177 (2010). doi:10.1016/j.abb.2009.11.029

    Article  CAS  PubMed  Google Scholar 

  13. Y. Wang, H.F. DeLuca, Is the vitamin D receptor found in muscle? Endocrinology 152(2), 354–363 (2011). doi:10.1210/en.2010-1109

    Article  CAS  PubMed  Google Scholar 

  14. Y. Wang, J. Zhu, H.F. DeLuca, Where is the vitamin D receptor? Arch. Biochem. Biophys. 523(1), 123–133 (2012). doi:10.1016/j.abb.2012.04.001

    Article  CAS  PubMed  Google Scholar 

  15. F.M. Damiani, R.M. Martin, A.C. Latronico, B. Ferraz-de-Souza, Normal bone mass and normocalcemia in adulthood despite homozygous vitamin D receptor mutations. Osteoporos. Int. 26(6), 1819–1823 (2015). doi:10.1007/s00198-015-3076-3

    Article  CAS  PubMed  Google Scholar 

  16. B. Ferraz-de-Souza, P.L.F. Costa, M.L. Katayama, E.T. Carneiro, M.A. Folgueira, M.M. França, Opposing vitamin D receptor activity in the absence of 1,25-dihydroxyvitamin D. (2016 submitted).

  17. L.T. Campos, H. Brentani, R.A. Roela, M.L. Katayama, L. Lima, C.F. Rolim, C. Milani, M.A. Folgueira, M.M. Brentani, Differences in transcriptional effects of 1α,25 dihydroxyvitamin D3 on fibroblasts associated to breast carcinomas and from paired normal breast tissues. J. Steroid Biochem. Mol. Biol. 133, 12–24 (2013). doi:10.1016/j.jsbmb.2012.08.002

    Article  CAS  PubMed  Google Scholar 

  18. B. Ferraz-de-Souza, F. Martin, D. Mallet, R.E. Hudson-Davies, P. Cogram, L. Lin, D. Gerrelli, F. Beuschlein, Y. Morel, A. Huebner, J.C. Achermann, CBP/p300-interacting transactivator, with Glu/Asp-rich C-terminal domain, 2, and pre-B-cell leukemia transcription factor 1 in human adrenal development and disease. J. Clin. Endocrinol. Metab. 94(2), 678–683 (2009). doi:10.1210/jc.2008-1064

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the Sao Paulo Research Foundation (Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP) Young Investigator grant number 2011/12696-4 (to B.F.-d.-S.) and the FAPESP scientific elective scholarship IC 2014/03561-6 (to P.L.F.C). We are grateful to Maria Lucia Katayama (LIM-24, FMUSP) for kindly providing CYP24A1 oligonucleotides and to Rodrigo Talada Iborra and team at LIM-10, HCFMUSP, for technical assistance with immunoblot imaging.

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Correspondence to Bruno Ferraz-de-Souza.

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Costa, P.L.F., França, M.M. & Ferraz-de-Souza, B. Nonspecific binding of a frequently used vitamin D receptor (VDR) antibody: important implications for vitamin D research in human health. Endocrine 54, 556–559 (2016). https://doi.org/10.1007/s12020-016-1036-0

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